Mahendran G, Narmatha Bai V
Plant Biotechnology Laboratory, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India.
Plant Tissue Culture Laboratory, Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore 641046, India.
J Genet Eng Biotechnol. 2016 Jun;14(1):77-81. doi: 10.1016/j.jgeb.2015.11.003. Epub 2015 Dec 31.
A protocol for induction of direct somatic embryogenesis and subsequent plant regeneration for the medicinally important and endangered plant of has been developed for the first time. In the present study, seed derived protocorm explants were cultured on half strength Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), Picloram and Dicamba individually and in combination with cytokinins BAP, TDZ and Kn for its effectiveness to induce the differentiation of somatic embryos. The best response was observed in protocorms cultured half strength MS medium supplemented with 2,4-D at 3.39 μM and TDZ at 6.80 μM. Both epidermal and sub epidermal cells were involved in the formation of embryos. The proembryos developed into globular stage and subsequently developed into protocoms. Complete plantlets were formed after 60 days of culture. The plantlets were acclimatized in plastic pots containing sterilized vermiculite. The survival rate was 76%.
首次开发了一种用于诱导重要药用濒危植物直接体细胞胚胎发生及后续植株再生的方案。在本研究中，将种子来源的原球茎外植体分别接种在添加了2,4 - 二氯苯氧乙酸（2,4 - D）、毒莠定和麦草畏，以及与细胞分裂素苄氨基嘌呤（BAP）、噻苯隆（TDZ）和激动素（Kn）组合的1/2强度Murashige和Skoog（MS）培养基上，以研究其诱导体细胞胚胎分化的效果。在添加3.39 μM 2,4 - D和6.80 μM TDZ的1/2强度MS培养基上培养的原球茎中观察到最佳反应。胚胎形成涉及表皮和亚表皮细胞。原胚发育成球形阶段，随后发育成原球茎。培养60天后形成完整植株。将植株在装有灭菌蛭石的塑料盆中驯化。成活率为76%。
