Hoti S L, Thangadurai R, Patra K P, Das P K
Molecular Biology and Bioinformatics Unit, Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry 605 006, India.
Infect Genet Evol. 2007 Mar;7(2):155-60. doi: 10.1016/j.meegid.2006.07.006. Epub 2006 Sep 12.
Nematode polyprotein allergens (NPA) are lipid binding/transport molecules that elicit elevated levels of IgE response in the infected host, leading to Th2 type of immune response. They also transport arachidonic acid and its metabolites that are known to be involved in the action of antifilarial drug, Diethylcarbamazine and hence are of great significance for the control of lymphatic filariasis. We investigated the polymorphism of gp15/400 polyprotein of 35 isolates of lymphatic filarial parasite Wuchereria bancrofti collected from different geographic locations of India. The repeat sub-unit of the gene was found to be highly conserved in all the isolates with only two nucleotide synonymous changes at positions 286 (A-G) and 337 (C-T). Since this molecule is highly conserved and has multifarious roles in the survival and pathogenesis of the parasite it has good potential as a target for drug, immunodulation tool and immunotherapy development.
线虫多聚蛋白变应原（NPA）是脂质结合/转运分子，可在受感染宿主中引发升高的IgE反应水平，导致Th2型免疫反应。它们还转运花生四烯酸及其代谢产物，已知这些物质参与抗丝虫药物乙胺嗪的作用，因此对于控制淋巴丝虫病具有重要意义。我们研究了从印度不同地理位置收集的35株淋巴丝虫寄生虫班氏吴策线虫的gp15/400多聚蛋白的多态性。发现该基因的重复亚基在所有分离株中高度保守，仅在第286位（A-G）和第337位（C-T）有两个核苷酸同义变化。由于该分子高度保守，并且在寄生虫的生存和发病机制中具有多种作用，因此它作为药物靶点、免疫调节工具和免疫治疗开发具有良好的潜力。
