Centre for Biotechnology, Anna University, Chennai, India.
Exp Parasitol. 2010 Jul;125(3):236-43. doi: 10.1016/j.exppara.2010.01.027. Epub 2010 Feb 6.
Wb14 of Wuchereria bancrofti, an orthologue of Brugia malayi SXP-1 and W. bancrofti SXP-1, was amplified from genomic DNA of W. bancrofti microfilaria collected from four distant geographical locations in India viz., Vellore, Bhubaneshwar, Pondicherry and Sevagram. The gene was sub-cloned in a prokaryotic vector pRSET and expressed in Escherichia coli as a truncated protein (approximately 23kDa). The nucleotide sequence of the gene is 98% similar to that of WbSXP-1 and is found to be intron-less. However, the analysis and comparison of the derived amino acid sequence with WbSXP-1 showed that Wb14 is truncated at amino acid position 153. The distribution of the two genes in the studied four geographical locations indicated that WbSXP-1 is prevalent only in parasite samples from Sevagram while Wb14 is present in parasites from all the other locations. Only a limited polymorphism was observed in both the genes among the parasites from different geographical locations.
从印度四个不同地理位置（Vellore、Bhubaneshwar、Pondicherry 和 Sevagram）采集的班氏丝虫微丝蚴基因组 DNA 中扩增了 Wuchereria bancrofti 的 Wb14，这是 Brugia malayi SXP-1 和 W. bancrofti SXP-1 的同源物。该基因在原核载体 pRSET 中进行亚克隆，并在大肠杆菌中表达为截断蛋白（约 23kDa）。该基因的核苷酸序列与 WbSXP-1 相似，相似度为 98%，并且被发现不含内含子。然而，对推导的氨基酸序列与 WbSXP-1 的分析和比较表明，Wb14 在氨基酸位置 153 处截断。这两个基因在研究的四个地理位置的分布表明，WbSXP-1 仅存在于 Sevagram 的寄生虫样本中，而 Wb14 存在于所有其他位置的寄生虫中。来自不同地理位置的寄生虫中，这两个基因仅观察到有限的多态性。
