Janardhan S, Pandiaraja P, Pandey V, Karande A, Kaliraj P
Centre for Biotechnology, Anna University, Chennai 600025, India.
J Helminthol. 2011 Mar;85(1):1-6. doi: 10.1017/S0022149X10000118. Epub 2010 Mar 26.
The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the World Health Organization. Presently, few immunodiagnostics are available for filarial monitoring programmes. The Wuchereria bancrofti (Wb) SXP-1 parasite protein, with 84% homology to Brugia malayi (Bm) SXP-1, was found to be highly immunogenic. WbSXP-1 is one among the diagnostic candidate molecules that were used for developing a rapid-antibody-flow-through diagnostic kit for filariasis. Studies were initiated with the aim of developing monoclonal antibodies against recombinant WbSXP-1 and prospective applications for the detection of both circulating Wb and Bm antigens in serum samples from infected individuals. The monoclones 1A6C2 of subclass IgG1k, and 2A12F8 of class IgM, specifically detected Wb and Bm microfilaria isolated from patients and did not show cross-reactivity with other filarial recombinant antigens. We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.
世界卫生组织强调了开发有效检测方法以诊断、监测和评估人类淋巴丝虫病的重要性。目前，用于丝虫监测项目的免疫诊断方法很少。已发现与马来布鲁线虫（Bm）SXP-1具有84%同源性的班氏吴策线虫（Wb）SXP-1寄生虫蛋白具有高度免疫原性。WbSXP-1是用于开发丝虫病快速抗体流通诊断试剂盒的诊断候选分子之一。研究旨在开发针对重组WbSXP-1的单克隆抗体，并探索其在检测感染个体血清样本中循环的Wb和Bm抗原方面的潜在应用。IgG1k亚类的单克隆抗体1A6C2和IgM类的单克隆抗体2A12F8能特异性检测从患者体内分离出的Wb和Bm微丝蚴，且与其他丝虫重组抗原无交叉反应。我们预计这项工作将解决发展中国家流行地区人类淋巴丝虫病快速诊断中面临的问题。
