Suenami Koichi, Lim Lee Wah, Takeuchi Toyohide, Sasajima Yasuhide, Sato Kiyohito, Takekoshi Yuji, Kanno Susumu
Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 1;846(1-2):176-83. doi: 10.1016/j.jchromb.2006.08.041. Epub 2006 Sep 12.
A rapid and sensitive analytical method has been developed for the simultaneous determination of 16 non-steroidal anti-inflammatory drugs (NSAIDs) in human plasma by capillary liquid chromatography (LC) and quadrupole mass spectrometry with electrospray ionization operated in the negative ion mode. The sample clean-up and enrichment on a pre-column were accomplished on-line to improve the sensitivity. This method greatly reduced sample preparation time and sample volume compared with off-line sample extraction methods and conventional LC methods, respectively. The recoveries of NSAIDs from human plasma were 56.7-96.9%. The total analytical time for a single analytical run was approximately 15 min. The detection limits of NSAIDs were 0.001-0.075 microg ml(-1) using a selected ion monitoring mode.
已开发出一种快速灵敏的分析方法，用于通过毛细管液相色谱（LC）和在负离子模式下运行的电喷雾电离四极杆质谱法同时测定人血浆中的16种非甾体抗炎药（NSAIDs）。通过在线方式完成柱前样品净化和富集，以提高灵敏度。与离线样品萃取方法和传统LC方法相比，该方法分别大大减少了样品制备时间和样品体积。NSAIDs在人血浆中的回收率为56.7 - 96.9%。单次分析运行的总分析时间约为15分钟。在选择离子监测模式下，NSAIDs的检测限为0.001 - 0.075微克/毫升。
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