采用固相萃取-液相色谱-质谱联用技术快速同时测定人血浆中的非甾体抗炎药
Rapid and simultaneous determination of nonsteroidal anti-inflammatory drugs in human plasma by LC-MS with solid-phase extraction.
作者信息
Suenami Koichi, Lim Lee Wah, Takeuchi Toyohide, Sasajima Yasuhide, Sato Kiyohito, Takekoshi Yuji, Kanno Susumu
机构信息
Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
出版信息
Anal Bioanal Chem. 2006 Apr;384(7-8):1501-5. doi: 10.1007/s00216-006-0352-2. Epub 2006 Mar 14.
A rapid, selective and sensitive analytical method for the simultaneous determination of 16 nonsteroidal anti-inflammatory drugs (NSAIDs) in human plasma was carried out using Oasis HLB solid-phase extraction (SPE), followed by reversed-phase high-performance liquid chromatography and quadrupole mass spectrometry with electrospray ionization operated in the negative ion mode. The recoveries of NSAIDs from human plasma by SPE were greater than 76.7%. The use of a short column packed with small particles enabled rapid and simultaneous determination within 7 min. The detection limits for the NSAIDs were 0.01-0.9 mug/ml using the selected ion monitoring mode.
采用Oasis HLB固相萃取（SPE），随后进行反相高效液相色谱和在负离子模式下运行的电喷雾电离四极杆质谱分析，建立了一种快速、选择性和灵敏的同时测定人血浆中16种非甾体抗炎药（NSAIDs）的分析方法。通过SPE从人血浆中回收NSAIDs的回收率大于76.7%。使用填充小颗粒的短柱能够在7分钟内快速同时测定。采用选择离子监测模式时，NSAIDs的检测限为0.01 - 0.9μg/ml。
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