Gore S, Kastan M, Civin C
Johns Hopkins Oncology Center, Baltimore, Maryland.
Bone Marrow Transplant. 1990 Jul;6 Suppl 1:39-41.
Recent advances in preparation of cells for flow cytometric analysis have enabled the sensitive detection of intracellular antigens. We have examined the utility of two color flow cytometry for the detection of minimal residual T cell acute lymphoblastic leukemia (T-ALL) using a combination of expression of the pan-T cell marker CD5 and intranuclear terminal deoxynucleotidyl transferase (TdT). CD5+TdT+ cells can be sensitively detected above background in remission bone marrows (0.03% sensitivity). Possible extension of this technique to the detection of minimal residual B-lineage ALL, acute myelogenous leukemia (AML), and non-hematologic malignancies is discussed.
流式细胞术分析细胞制备方面的最新进展使得细胞内抗原的灵敏检测成为可能。我们使用全T细胞标志物CD5和核内末端脱氧核苷酸转移酶（TdT）的联合表达，研究了双色流式细胞术检测微小残留T细胞急性淋巴细胞白血病（T-ALL）的效用。在缓解期骨髓中，CD5+TdT+细胞能够在背景之上被灵敏地检测到（灵敏度为0.03%）。本文还讨论了将该技术扩展用于检测微小残留B系ALL、急性髓细胞白血病（AML）及非血液系统恶性肿瘤的可能性。
