Knapp W, Strobl H, Majdic O
Institut für Immunologie der Universität Wien, Austria.
Cytometry. 1994 Dec 15;18(4):187-98. doi: 10.1002/cyto.990180402.
New technology allows highly sensitive flow cytometric detection and quantitative analysis of intracellular antigens in normal and malignant hemopoietic cells. With this technology, the earliest stages of myeloid and lymphoid differentiation can easily and reliably be identified using antibodies directed against (pro-)myeloperoxidase/MPO, CD22 and CD3 antigens, respectively. Particularly for the analysis of undifferentiated acute myeloblastic leukemia (AML) cells, the immunological demonstration of intracellular MPO or its enzymatically inactive proforms is highly relevant, since other myeloid marker molecules such as CD33, CD13, or CDw65 are either not restricted to the granulomonocytic lineage or appear later in differentiation. By combining MPO staining with staining for lactoferrin (LF), undifferentiated cells can be distinguished from the granulomonocytic maturation compartment in bone marrow, since LF is selectively expressed from the myelocyte stage of differentiation onward. The list of informative intracellular antigens to be used in leukemia cell analysis will certainly expand in the near future. One candidate, intracellular CD68, has already been tested by us, and results are presented. Also dealt within this article are surface marker molecules not (as yet) widely used in leukemia cell analysis but with the potential to provide important additional information. Among them are the surface structures CD15, CD15s, CDw65, CD79a (MB-1), CD79b (B29), CD87 (uPA-R), and CD117 (c-kit).
新技术可实现对正常和恶性造血细胞内抗原进行高灵敏度的流式细胞术检测及定量分析。利用该技术，分别使用针对（原）髓过氧化物酶/MPO、CD22和CD3抗原的抗体，可轻松且可靠地识别髓系和淋巴系分化的最早阶段。特别是对于未分化急性髓性白血病（AML）细胞的分析，细胞内MPO或其无酶活性的前体形式的免疫证明具有高度相关性，因为其他髓系标志物分子，如CD33、CD13或CDw65，要么不限于粒单核细胞系，要么在分化后期才出现。通过将MPO染色与乳铁蛋白（LF）染色相结合，可将未分化细胞与骨髓中的粒单核细胞成熟区室区分开来，因为LF从分化的髓细胞阶段开始选择性表达。用于白血病细胞分析的信息丰富的细胞内抗原清单在不久的将来肯定会扩大。我们已经测试了一个候选物，细胞内CD68，并展示了结果。本文还讨论了尚未在白血病细胞分析中广泛使用但有可能提供重要额外信息的表面标志物分子。其中包括表面结构CD15、CD15s、CDw65、CD79a（MB-1）、CD79b（B29）、CD87（uPA-R）和CD117（c-kit）。
