Ohta Aki, Ebihara Nobuyuki, Hiratsuka Yoshimune, Yokoyama Toshiyuki, Murakami Akira, Oshimi Kazuo
Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.
Nippon Ganka Gakkai Zasshi. 2006 Aug;110(8):588-93.
We investigated whether vitreous cytology and the measurement of intravitreous cytokine were useful for the diagnosis of intraocular malignant lymphoma.
8 eyes of 5 patients with suspected intraocular malignant lymphoma during the past 15 months. 3 vitreous samples were collected from 3 patients at the time of pars plana vitrectomy. Polymerase chain reaction(PCR) amplification and flowcytometric analysis(FACS) of the vitreous samples were performed. Interleukin (IL)-10 and IL-6 concentrations were measured.
Vitreous cytology showed increased atypical B lymphocytes. The vitreous IL-10/IL-6 ratio was higher than 1 in all cases. Monoclonal rearrangement of the immunoglobulin heavy chain gene and the light chain restriction of immunoglobulin were detected.
The detection of the monoclonality of infiltrated cells into the vitreous by PCR amplification and FACS, and the measurements of IL-10 and IL-6 concentrations in the vitreous fluid may be useful in the diagnosis of intraocular malignant lymphoma.
我们研究了玻璃体细胞学检查及玻璃体内细胞因子测定对眼内恶性淋巴瘤诊断是否有用。
在过去15个月中，对5例疑似眼内恶性淋巴瘤患者的8只眼进行研究。在3例患者行扁平部玻璃体切除术时采集3份玻璃体样本。对玻璃体样本进行聚合酶链反应（PCR）扩增和流式细胞术分析（FACS）。测定白细胞介素（IL）-10和IL-6浓度。
玻璃体细胞学检查显示非典型B淋巴细胞增多。所有病例中玻璃体内IL-10/IL-6比值均高于1。检测到免疫球蛋白重链基因的单克隆重排及免疫球蛋白轻链限制。
通过PCR扩增和FACS检测玻璃体内浸润细胞的单克隆性，以及测定玻璃体液中IL-10和IL-6浓度，可能有助于眼内恶性淋巴瘤的诊断。
