Schittenhelm J, Mittelbronn M, Roser F, Tatagiba M, Mawrin C, Bornemann A
Institute of Brain Research, University of Tübingen, Tübingen, Germany.
Neuropathol Appl Neurobiol. 2006 Oct;32(5):525-31. doi: 10.1111/j.1365-2990.2006.00761.x.
The matricellular glycoprotein SPARC (secreted protein, acidic and rich in cysteine), also termed osteonectin, has been found to regulate the invasive behaviour of several tumour types by interacting with basement membrane constituents. Brain invasive meningiomas are supposed to disrupt the pial-glial basement membrane. In the present study we aimed at determining the relationship of basement membrane intactness and SPARC protein expression at the meningioma-brain border. Sections of 51 brain-invasive meningiomas (31 meningothelial meningiomas WHO grade I, 11 atypical WHO grade II, and nine anaplastic WHO grade III tumours) were immunolabelled with antibodies against SPARC, epithelial membrane antigen (EMA), collagen IV and glial fibrillary acidic protein (GFAP). Twenty-two non-invasive WHO grade I meningothelial meningiomas were included in the study for comparison. At the tumour-brain border of invasive meningiomas, spindle-shaped tumour cells expressed SPARC. The number of tumours containing SPARC+ spindle cells did not differ significantly between WHO grades. By contrast, the number of WHO grade I tumours expressing collagen IV (15/31) was highly significantly elevated when compared with WHO grade II (1/11) and WHO grade III (0/9) (both P < 0.0001). There was an inverse relationship of the presence of SPARC+ spindle cells and basement membrane material. In conclusion, the destruction of the basement membrane is correlated with meningioma malignancy grade whereas the expression of SPARC protein at the tumour-brain border is not. Destruction of the basement membrane and appearance of SPARC+ spindle cells are not coincident during the course of brain invasion by meningiomas.
基质细胞糖蛋白SPARC（分泌性蛋白，酸性且富含半胱氨酸），也被称为骨连接蛋白，已被发现可通过与基底膜成分相互作用来调节多种肿瘤类型的侵袭行为。脑侵袭性脑膜瘤被认为会破坏软脑膜-胶质基底膜。在本研究中，我们旨在确定脑膜瘤-脑边界处基底膜完整性与SPARC蛋白表达之间的关系。对51例脑侵袭性脑膜瘤（31例WHO I级脑膜内皮型脑膜瘤、11例WHO II级非典型脑膜瘤和9例WHO III级间变性肿瘤）的切片进行免疫标记，使用抗SPARC、上皮膜抗原（EMA）、IV型胶原和胶质纤维酸性蛋白（GFAP）的抗体。22例非侵袭性WHO I级脑膜内皮型脑膜瘤被纳入研究以作比较。在侵袭性脑膜瘤的肿瘤-脑边界处，梭形肿瘤细胞表达SPARC。SPARC+梭形细胞的肿瘤数量在WHO分级之间无显著差异。相比之下，与WHO II级（1/11）和WHO III级（0/9）相比，表达IV型胶原的WHO I级肿瘤数量（15/31）显著升高（均P<0.0001）。SPARC+梭形细胞的存在与基底膜物质呈负相关。总之，基底膜的破坏与脑膜瘤恶性程度相关，而肿瘤-脑边界处SPARC蛋白的表达则不然。在脑膜瘤脑侵袭过程中，基底膜的破坏与SPARC+梭形细胞的出现并不一致。
