Li Robert W, Li CongJun
Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture-Agricultural Research Service, Beltsville, MD 20705, USA.
BMC Genomics. 2006 Sep 14;7:234. doi: 10.1186/1471-2164-7-234.
Global gene expression profiles of bovine kidney epithelial cells regulated by sodium butyrate were investigated with high-density oligonucleotide microarrays. The bovine microarray with 86,191 distinct 60mer oligonucleotides, each with 4 replicates, was designed and produced with Maskless Array Synthesizer technology. These oligonucleotides represent approximately 45,383 unique cattle sequences.
450 genes significantly regulated by butyrate with a median False Discovery Rate (FDR) = 0 % were identified. The majority of these genes were repressed by butyrate and associated with cell cycle control. The expression levels of 30 selected genes identified by the microarray were confirmed using real-time PCR. The results from real-time PCR positively correlated (R = 0.867) with the results from the microarray.
This study presented the genes related to multiple signal pathways such as cell cycle control and apoptosis. The profound changes in gene expression elucidate the molecular basis for the pleiotropic effects of butyrate on biological processes. These findings enable better recognition of the full range of beneficial roles butyrate may play during cattle energy metabolism, cell growth and proliferation, and possibly in fighting gastrointestinal pathogens.
利用高密度寡核苷酸微阵列研究丁酸钠调控的牛肾上皮细胞的全基因组表达谱。采用无掩膜阵列合成技术设计并制作了包含86,191个不同的60聚体寡核苷酸的牛微阵列，每个寡核苷酸有4个重复。这些寡核苷酸代表了约45,383个独特的牛序列。
鉴定出450个受丁酸盐显著调控的基因，其中位错误发现率（FDR）= 0%。这些基因中的大多数被丁酸盐抑制，并与细胞周期调控相关。使用实时PCR确认了微阵列鉴定出的30个选定基因的表达水平。实时PCR的结果与微阵列的结果呈正相关（R = 0.867）。
本研究展示了与细胞周期调控和凋亡等多种信号通路相关的基因。基因表达的深刻变化阐明了丁酸盐对生物学过程多效性作用的分子基础。这些发现有助于更好地认识丁酸盐在牛能量代谢、细胞生长和增殖过程中以及可能在对抗胃肠道病原体方面可能发挥的全部有益作用。
