Sekiguchi J, Ohsaki T, Yamamoto H, Koichi K, Shida T
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
J Gen Microbiol. 1990 Mar;136(3):535-43. doi: 10.1099/00221287-136-3-535.
Mitochondrial DNA (mt DNA) from a patulin producer, Penicillium urticae (synonym P. griseofulvum), was 27.8 kb +/- 0.6 kb in size by electron microscopy and 27.2 kb by agarose gel electrophoresis. Restriction endonuclease maps for nine restriction enzymes were constructed, and eleven fragments which covered the total range of the mt DNA were cloned into the Escherichia coli plasmid vector pUC19. Southern analysis of the native genomes of P. urticae and P. chrysogenum with six of the cloned fragments as probes indicated similar genome arrangements as well as similar restriction maps. Both the large and small rRNA genes of P. urticae and P. chrysogenum were located on these restriction maps using Southern hybridization, and the result also supported the similar arrangement. Agarose/formaldehyde gel electrophoresis indicated that the small rRNA was 1.5 kb in size in both species; but, surprisingly, the large rRNA was 4.2 kb in size for P. urticae and 3.5 kb for P. chrysogenum. These sizes were, respectively, 1.1 kb and 0.4 kb larger than those from the very closely related Aspergillus nidulans.
通过电子显微镜观察，来自展青霉素产生菌荨麻青霉（同义词：灰黄青霉）的线粒体DNA（mt DNA）大小为27.8 kb±0.6 kb，而通过琼脂糖凝胶电泳测定其大小为27.2 kb。构建了9种限制酶的限制酶切图谱，并将覆盖mt DNA整个范围的11个片段克隆到大肠杆菌质粒载体pUC19中。以6个克隆片段为探针，对荨麻青霉和产黄青霉的天然基因组进行Southern分析，结果表明它们的基因组排列和限制酶切图谱相似。利用Southern杂交技术，在这些限制酶切图谱上定位了荨麻青霉和产黄青霉的大、小rRNA基因，结果也支持了它们相似的排列方式。琼脂糖/甲醛凝胶电泳表明，两种菌的小rRNA大小均为1.5 kb；但令人惊讶的是，荨麻青霉的大rRNA大小为4.2 kb，产黄青霉的大rRNA大小为3.5 kb。这些大小分别比与其亲缘关系很近的构巢曲霉的大rRNA大1.1 kb和0.4 kb。
