包含构巢曲霉5S核糖体RNA基因的14个不同DNA片段的克隆与定位

Cloning and mapping of fourteen different DNA fragments containing Aspergillus nidulans 5S rRNA genes.

作者信息

Bartnik E, Kulik J, Nagieć M M, Zagrodzka M

出版信息

Acta Microbiol Pol. 1984;33(1):5-10.

Abstract

Fourteen different plasmids hybridizing to Aspergillus nidulans 5S rRNA were isolated from a gene bank obtained after cloning Sau3A partial digests of A. nidulans DNA in a yeast--Escherichia coli vector, pBB29. The restriction maps of these plasmids were determined. The size of the cloned fragments was 2.7-9.5 kb, 12 of the plasmids were found to code for single 5S rRNA genes and 2 coded for 2 genes. No similarity of the sequences surrounding the 5S rRNA genes was found by restriction mapping.

摘要

从一个基因文库中分离出了14种与构巢曲霉5S rRNA杂交的不同质粒，该基因文库是在将构巢曲霉DNA的Sau3A部分消化产物克隆到酵母-大肠杆菌载体pBB29中后获得的。确定了这些质粒的限制性图谱。克隆片段的大小为2.7 - 9.5 kb，发现其中12个质粒编码单个5S rRNA基因，2个编码2个基因。通过限制性图谱分析未发现5S rRNA基因周围序列的相似性。

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