Hu Bin, Gong Qianhong, Wang Ye, Ma Yiming, Li Jingbao, Yu Wengong
Key Laboratory of Marine Drugs of Ministry of Education, Marine Drug and Food Institute, Ocean University of China, Qingdao 266003, China.
Anaerobe. 2006 Oct-Dec;12(5-6):260-6. doi: 10.1016/j.anaerobe.2006.07.005. Epub 2006 Sep 14.
To investigate the prebiotic properties of neoagaro-oligosaccharides (NAOS), obtained from enzymatic hydrolysis of agarose, the in vitro and in vivo effects of NAOS on bacterial growth were studied. In vitro NAOS were found to be highly resistant to enzymes of the upper gastrointestinal tract, which remained intact after 24h incubation with different amylolytic enzymes. NAOS significantly stimulated the growth of bifidobacteria and lactobacilli in Man-Rogosa-Sharp (MRS) medium, anaerobically. Compared with fructo-oligosaccharides (FOS), 1% (w/v) NAOS promoted the specific growth rate of beneficial bacteria by about 100%. The decreases of media pH with NAOS were almost the same as that with FOS. In vivo, NAOS significantly increased the numbers of lactobacilli and bifidobacteria (P<0.05) in fresh feces or cecal content while reducing putrefactive microorganisms. Mice fed with 2.5% (w/v) NAOS for 7 days had larger increases in colonic beneficial bacteria population than those fed with even 5% (w/v) FOS for 14 days. No side effects, such as eructation and bloating, were found. Interestingly, NAOS with higher degrees of polymerization (DP) showed better prebiotic activity. These results indicated that NAOS had great prebiotic effect, which could be beneficial to the host.
为研究从琼脂糖酶解获得的新琼脂寡糖（NAOS）的益生元特性，对NAOS在体外和体内对细菌生长的影响进行了研究。体外实验发现，NAOS对上消化道的酶具有高度抗性，在与不同淀粉酶孵育24小时后仍保持完整。在厌氧条件下，NAOS在曼-罗戈萨-夏普（MRS）培养基中显著刺激双歧杆菌和乳酸杆菌的生长。与低聚果糖（FOS）相比，1%（w/v）的NAOS使有益细菌的比生长速率提高了约100%。NAOS导致培养基pH值的下降与FOS几乎相同。在体内，NAOS显著增加了新鲜粪便或盲肠内容物中乳酸杆菌和双歧杆菌的数量（P<0.05），同时减少了腐败微生物。喂食2.5%（w/v）NAOS 7天的小鼠结肠有益细菌数量的增加幅度大于喂食5%（w/v）FOS 14天的小鼠。未发现诸如嗳气和腹胀等副作用。有趣的是，聚合度（DP）较高的NAOS表现出更好的益生元活性。这些结果表明，NAOS具有很强的益生元效应，对宿主有益。
