Ouyang Jia, Wang Feng, Li Wenjia, Li Qingming, Su Xiaojun
College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Hunan Provincial Research Center of Engineering and Technology for Fermented Food, Changsha 410128, China.
Foods. 2021 Nov 4;10(11):2698. doi: 10.3390/foods10112698.
To clarify the mechanisms underlying the growth-promoting effects of yam polysaccharide on , the yam polysaccharide was extracted using a deep eutectic solvents (DESs) method and separated into four fractions by DEAE-cellulose 52. These fractions were used as the alternative carbon source to substitute lactose to compare their growth-promoting effects on . Furthermore, their molecular weight, monosaccharide and functional groups' composition, microscopic forms and other basic structure characterizations were analyzed. The results showed that all the fractions could significantly promote growth, and fractions exhibited significantly different growth-promoting effects, whose viable count increased by 6.14, 6.03, 11.48 and 11.29%, respectively, relative to those in the M17 broth medium. Structure-activity relationship analysis revealed that the high growth-promoting activity of yam polysaccharide might be more dependent on the higher molecular weight, the higher galacturonic acid content and its complex spatial configuration, and the existence of β-glycosides would make the yam polysaccharide have a better growth-promoting effect on .
为阐明山药多糖对[具体对象]生长促进作用的潜在机制，采用深共熔溶剂（DESs）法提取山药多糖，并通过DEAE - 纤维素52将其分离为四个级分。这些级分用作替代碳源来替代乳糖，以比较它们对[具体对象]的生长促进作用。此外，分析了它们的分子量、单糖和官能团组成、微观形态及其他基本结构特征。结果表明，所有级分均能显著促进[具体对象]生长，且各分数级分表现出显著不同的生长促进效果，相对于M17肉汤培养基中的[具体对象]，其活菌数分别增加了6.14%、6.03%、11.48%和11.29%。构效关系分析表明，山药多糖的高生长促进活性可能更依赖于较高的分子量、较高的半乳糖醛酸含量及其复杂的空间构型，并且β - 糖苷的存在会使山药多糖对[具体对象]具有更好的生长促进作用。
