Laviada M D, Videgain S P, Moreno L, Alonso F, Enjuanes L, Escribano J M
Instituto Nacional de Investigaciones Agrarias, Departamento de Sanidad Animal, Madrid, Spain.
Virus Res. 1990 Jul;16(3):247-54. doi: 10.1016/0168-1702(90)90051-c.
The peplomer protein (S) and the transmembrane protein (M) of transmissible gastroenteritis virus (TGEV) of swine were identified by iodination and serologically on the surface of infected cells. Of a total of 4 monoclonal antibodies (mAb) directed against four antigenic sites of S protein (Correa et al., 1988), 3 specific for sites A, B and D attached to the plasma membrane of infected cells, as disclosed by indirect immunofluorescence and by complement-mediated cytolysis. Four of the mAbs assayed were specific for the viral protein M and two of them gave plasma membrane immunofluorescence and mediated cytolysis in the presence of complement. The viral nucleoprotein N could not be demonstrated on the surface of infected cells either by iodination or employing 3 mAbs against this protein. Finally, a time course infection experiment demonstrated that S and M proteins were expressed on the surface of infected cells at 4 h after infection, before infective virus was released from infected cells.
通过碘化法和血清学方法，在感染细胞表面鉴定了猪传染性胃肠炎病毒（TGEV）的纤突蛋白（S）和跨膜蛋白（M）。针对S蛋白四个抗原位点的总共4种单克隆抗体（mAb）（Correa等人，1988年），其中3种对位点A、B和D具有特异性，通过间接免疫荧光和补体介导的细胞溶解显示，它们附着在感染细胞的质膜上。所检测的mAb中有4种对病毒蛋白M具有特异性，其中2种在有补体存在的情况下产生质膜免疫荧光并介导细胞溶解。无论是通过碘化法还是使用针对该蛋白的3种mAb，均未在感染细胞表面证实病毒核蛋白N。最后，一项时间进程感染实验表明，在感染性病毒从感染细胞中释放之前，感染后4小时S和M蛋白就在感染细胞表面表达。
