冠状病毒膜蛋白胞内结构域中一个免疫显性表位的鉴定
Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein.
作者信息
Dong Hui, Zhang Xin, Shi Hongyan, Chen Jianfei, Shi Da, Zhu Yunnuan, Feng Li
机构信息
State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Molecular Biology (Gembloux Agro-Bio Tech), University of Liège (ULg), Liège 4000, Belgium.
出版信息
Viruses. 2016 Dec 10;8(12):327. doi: 10.3390/v8120327.
The coronavirus membrane (M) protein acts as a dominant immunogen and is a major player in virus assembly. In this study, we prepared two monoclonal antibodies (mAbs; 1C3 and 4C7) directed against the transmissible gastroenteritis virus (TGEV) M protein. The 1C3 and 4C7 mAbs both reacted with the native TGEV M protein in western blotting and immunofluorescence (IFA) assays. Two linear epitopes, 243YSTEART249 (1C3) and 243YSTEARTDNLSEQEKLLHMV262 (4C7), were identified in the endodomain of the TGEV M protein. The 1C3 mAb can be used for the detection of the TGEV M protein in different assays. An IFA method for the detection of TGEV M protein was optimized using mAb 1C3. Furthermore, the ability of the epitope identified in this study to stimulate antibody production was also evaluated. An immunodominant epitope in the TGEV membrane protein endodomain was identified. The results of this study have implications for further research on TGEV replication.
冠状病毒膜（M）蛋白作为主要免疫原，在病毒组装中起主要作用。在本研究中，我们制备了两种针对传染性胃肠炎病毒（TGEV）M蛋白的单克隆抗体（mAb；1C3和4C7）。1C3和4C7单克隆抗体在蛋白质免疫印迹和免疫荧光（IFA）试验中均与天然TGEV M蛋白发生反应。在TGEV M蛋白的内结构域中鉴定出两个线性表位，即243YSTEART249（1C3）和243YSTEARTDNLSEQEKLLHMV262（4C7）。1C3单克隆抗体可用于不同试验中TGEV M蛋白的检测。使用单克隆抗体1C3优化了一种检测TGEV M蛋白的IFA方法。此外，还评估了本研究中鉴定的表位刺激抗体产生的能力。在TGEV膜蛋白内结构域中鉴定出一个免疫显性表位。本研究结果对TGEV复制的进一步研究具有启示意义。
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