Kanamori S, Urisu A, Iimi K, Kondo Y, Horiba F, Masuda S, Tsuruta M, Yazaki T
Department of Pediatrics, Fujita Health University, School of Medicine.
Arerugi. 1990 May;39(5):452-8.
Human recombinant interleukin-3 (hrIL-3) released histamine from human leukocytes in vitro. The histamine release by hrIL-3 significantly correlated with those by anti-IgE, thrombin, and f-met. peptide, but not by A23187. Histamine release by hrIL-3 was a Ca2(+)-dependent reaction, as was that by anti-IgE, although the time course of histamine release by hrIL-3 was slower than that by anti-IgE. Pre-treatment of leukocytes with hrIL-3 decreased the histamine release by hrIL-3 itself, but enhanced the histamine releases by anti-IgE, f-met. peptide, thrombin and A23187. These results suggested that the mechanism of hrIL-3-induced histamine release was different from those of anti-IgE, f-met. peptide, thrombin, and A23187. There was no significant difference between hrIL-3-induced histamine release of leukocytes from asthmatics and healthy controls.
人重组白细胞介素-3（hrIL-3）在体外可使人白细胞释放组胺。hrIL-3诱导的组胺释放与抗IgE、凝血酶和f-甲硫氨酸肽诱导的组胺释放显著相关，但与A23187诱导的组胺释放无关。hrIL-3诱导的组胺释放是一种Ca2(+)依赖性反应，抗IgE诱导的组胺释放也是如此，尽管hrIL-3诱导组胺释放的时间进程比抗IgE诱导的慢。用hrIL-3预处理白细胞可降低hrIL-3自身诱导的组胺释放，但可增强抗IgE、f-甲硫氨酸肽、凝血酶和A23187诱导的组胺释放。这些结果表明，hrIL-3诱导组胺释放的机制与抗IgE、f-甲硫氨酸肽、凝血酶和A23187诱导组胺释放的机制不同。哮喘患者和健康对照者的白细胞经hrIL-3诱导的组胺释放无显著差异。
