Di Carlo M, Montana G, Bonura A
Istituto di Biologia dello Sviluppo C.N.R., Palermo, Italy.
Mol Reprod Dev. 1990 Jan;25(1):28-36. doi: 10.1002/mrd.1080250106.
Two cDNA clones related to Paracentrotus lividus butanol-extracted proteins, presumably belonging to cell surface proteins, were isolated by a lambda gt11 expression library of ovary poly A+ RNA. These clones, called bep1 and bep4, of 1,110 and 1,071 bp, respectively, belong to a multigene family. By sequencing analysis, a special structural organization in the coding region is detected. A single copy region is inserted between two regions different from each other but similar in the two clones, which constitute two perfectly preserved domains in the genome and are not always present together in the various members of this gene family. The bep1 and bep4 clones derive from two single genes that are polymorphic in the sea urchin genome. Expression of these clones was studied by Northern blot analysis. Both bep1 and bep4 are transcribed during oogenesis into mRNAs of 1.4 kb, which are stored in eggs and utilized during early embryogenesis. None of these RNAs is, in fact, detectable after the gastrula stage. Moreover, the transcripts of three other members of the family are present in eggs and at the 32 cell stage, but they are also synthesized in the early developmental stages.
通过紫海胆卵巢多聚腺苷酸加尾RNA的λgt11表达文库，分离出了两个与紫海胆丁醇提取蛋白相关的cDNA克隆，推测它们属于细胞表面蛋白。这些分别为1110 bp和1071 bp的克隆，称为bep1和bep4，属于一个多基因家族。通过序列分析，在编码区检测到一种特殊的结构组织。一个单拷贝区域插入在两个彼此不同但在两个克隆中相似的区域之间，这两个区域在基因组中构成两个完美保存的结构域，并且在该基因家族的不同成员中并不总是同时出现。bep1和bep4克隆源自海胆基因组中两个多态性的单基因。通过Northern印迹分析研究了这些克隆的表达。bep1和bep4在卵子发生过程中均转录为1.4 kb的mRNA，这些mRNA存储在卵子中并在早期胚胎发育过程中被利用。事实上，在原肠胚阶段之后就检测不到这些RNA中的任何一种。此外，该家族的其他三个成员的转录本在卵子和32细胞阶段存在，但它们也在早期发育阶段合成。
