Di Carlo M, Romancino D P, Montana G, Ghersi G
Istituto di Biologia dello Sviluppo, Consiglio Nazionale delle Ricerche, Palermo, Italy.
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5622-6. doi: 10.1073/pnas.91.12.5622.
We demonstrated that two mRNAs that are synthesized during the vitellogenic period of oogenesis and that code for cell surface proteins are asymmetrically distributed in the unfertilized egg of Paracentrotus lividus. At fertilization, these RNAs rapidly localize in the cortical zone at the animal pole of the egg. They are then detected in the mesomeres and the macromeres, but not in the micromeres, and thereafter are found in the ectoderm but not in the vegetal plate, mesenchyme cells, or early intestine. They disappear in late gastrula. The proteins synthesized by these mRNAs show the same territorial location during the period examined here, which included the unfertilized egg and the 16-blastomere stage. These conclusions were reached on the basis of in situ hybridization and immunostaining experiments, as well as Northern and Western blot analyses of isolated blastomeres. The possible significance of this asymmetric distribution of these two mRNAs and proteins in the establishment of the animal/vegetal axis is discussed.
我们证明，在卵子发生的卵黄发生期合成的、编码细胞表面蛋白的两种mRNA，在紫球海胆未受精卵中呈不对称分布。受精时，这些RNA迅速定位在卵动物极的皮质区。然后在中裂球和大裂球中检测到它们，但在小裂球中未检测到，此后在外胚层中发现它们，但在植物极板、间充质细胞或早期肠道中未发现。它们在原肠胚后期消失。在本文研究的时期内，包括未受精卵和16细胞期，由这些mRNA合成的蛋白质显示出相同的区域定位。这些结论是基于原位杂交和免疫染色实验，以及对分离的卵裂球的Northern和Western印迹分析得出的。本文讨论了这两种mRNA和蛋白质的这种不对称分布在动物/植物轴建立中的可能意义。
