Eliason J A, Maurice D M
Department of Ophthalmology, Stanford University Medical Center, CA 94305.
Br J Ophthalmol. 1990 Sep;74(9):519-22. doi: 10.1136/bjo.74.9.519.
Both the distribution of tear fluid over the conjunctiva and any injury to the conjunctival epithelium can be made visible by instilling a fluorescent solution into the eye and observing with an appropriate combination of excitation and barrier filters. Sulphorhodamine B, which has an orange fluorescence that can be separated from the green natural fluorescence of the ocular tissues, gives a greater contrast than fluorescein. The tear film is seen to cover the surface of the conjunctiva and to be concentrated in its folds. Small circular areas of thin tear film appear transiently in the neighbourhood of the limbus after a blink. Occasional cells stain on the normal conjunctiva, particularly in the interpalpebral area. The density of the staining increases in dry eye conditions. Conjunctival trauma is sensitively revealed by the method, and its healing can be followed. Hard contact lenses are seen to traumatize continually the inferior limbal conjunctiva in symptomless wearers.
通过向眼睛滴入荧光溶液并使用合适的激发滤光片和阻挡滤光片组合进行观察，泪液在结膜上的分布以及结膜上皮的任何损伤都能显现出来。磺罗丹明B发出橙色荧光，可与眼组织的绿色自然荧光区分开，其对比度比荧光素更高。可以看到泪膜覆盖结膜表面并集中在其皱襞中。眨眼后，角膜缘附近会短暂出现薄泪膜的小圆形区域。正常结膜上偶尔会有细胞染色，尤其是在睑裂区。在干眼情况下，染色密度会增加。该方法能灵敏地显示结膜创伤，并可跟踪其愈合情况。在无症状佩戴者中，可以看到硬性隐形眼镜会持续损伤下角膜缘结膜。
