Fitzpatrick D R, Snider M, McDougall L, Beskorwayne T, Babiuk L A, Zamb T J, Bielefeldt Ohmann H
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Immunology. 1990 Aug;70(4):504-12.
Bovine herpes virus type 1 (BHV-1) gIII is a major virion glycoprotein with homology to the immunoglobulin superfamily. We have investigated the possibility that gIII is related to host molecules and have identified a gIII-specific monoclonal antibody (mAb) that cross-reacts with normal bovine cells. The cross-reactive entity was expressed mainly on monocyte/macrophages (M phi), polymorphonuclear leucocytes (PMN) and platelets, and was identified as a 43,000-63,000 molecular weight (MW) cell-surface glycoprotein. For M phi, the glycoprotein appears to be a general lineage marker, rather than a maturation or activation marker, and may be a functional receptor, as evidenced by its endocytosis via coated pits and its involvement in proliferation of mononuclear cells in vitro. This novel leucocyte marker was also detected on subsets of human, ovine and canine M phi. Competitive binding assays with sera from cattle immunized with BHV-1 or gIII revealed apparent low responsiveness to the cross-reactive epitope. The results suggest that BHV-1 gIII is antigenically related to a novel host leucocyte receptor and that evasion and/or interference with leucocyte function may be a consequence of this molecular mimicry relationship.
牛疱疹病毒1型（BHV - 1）的糖蛋白III（gIII）是一种主要的病毒体糖蛋白，与免疫球蛋白超家族具有同源性。我们研究了gIII与宿主分子相关的可能性，并鉴定出一种与正常牛细胞发生交叉反应的gIII特异性单克隆抗体（mAb）。这种交叉反应实体主要表达于单核细胞/巨噬细胞（M phi）、多形核白细胞（PMN）和血小板上，并被鉴定为一种分子量为43,000 - 63,000（MW）的细胞表面糖蛋白。对于M phi而言，该糖蛋白似乎是一种通用的谱系标志物，而非成熟或激活标志物，并且可能是一种功能性受体，通过其经有被小窝的内吞作用以及在体外参与单核细胞增殖可证明这一点。在人、绵羊和犬的M phi亚群上也检测到了这种新型白细胞标志物。用BHV - 1或gIII免疫的牛血清进行的竞争性结合试验显示，对交叉反应表位的反应明显较低。结果表明，BHV - 1 gIII在抗原性上与一种新型宿主白细胞受体相关，并且逃避和/或干扰白细胞功能可能是这种分子模拟关系的结果。
