Goyert S M, Ferrero E M, Seremetis S V, Winchester R J, Silver J, Mattison A C
J Immunol. 1986 Dec 15;137(12):3909-14.
Six monoclonal antibodies (MAb) which react with myelomonocytic cells representing various stages of differentiation, and which precipitate six different cell surface molecules, were identified. A 50 to 55 kilodalton (Kd) glycoprotein, restricted in expression to mature cells of the monocyte lineage, was detected by immunoprecipitation with antibody MoS39. By using COS-7 cells transfected with a cDNA clone encoding the MoS39 antigen, various well-described anti-monocyte MAb, including Mo2, My4, Leu-M3 (MoP9), MoP15, MoS1, and 63D3, also bound to MoS39-expressing COS-7 cells, suggesting that this group of antibodies reacted with the same glycoprotein. Immature cells of the myelomonocytic lineage were shown to express two distinct molecules: one with an m.w. of 26 to 28 Kd identified by antibody SG133, and the second, a 130 to 140 Kd glycoprotein identified by MoU26. Mature granulocytes were found to express a 60 Kd molecule identified by antibody SG185 which was absent from other cells of this lineage. Two other molecules were shown to be present on both mature and immature cells of the granulocytic and monocytic lineages: a 130 to 140 Kd glycoprotein identified by antibody SG134, and a 160 to 170 Kd glycoprotein recognized by antibody MoU48.
鉴定出六种单克隆抗体（MAb），它们与代表不同分化阶段的骨髓单核细胞发生反应，并沉淀出六种不同的细胞表面分子。通过用抗体MoS39进行免疫沉淀，检测到一种50至55千道尔顿（Kd）的糖蛋白，其表达仅限于单核细胞系的成熟细胞。通过使用用编码MoS39抗原的cDNA克隆转染的COS-7细胞，各种已充分描述的抗单核细胞MAb，包括Mo2、My4、Leu-M3（MoP9）、MoP15、MoS1和63D3，也与表达MoS39的COS-7细胞结合，这表明这组抗体与同一糖蛋白发生反应。骨髓单核细胞系的未成熟细胞显示表达两种不同的分子：一种分子量为26至28 Kd，由抗体SG133鉴定，另一种是130至140 Kd的糖蛋白，由MoU26鉴定。发现成熟粒细胞表达一种由抗体SG185鉴定的60 Kd分子，该分子在该细胞系的其他细胞中不存在。另外两种分子显示存在于粒细胞系和单核细胞系的成熟和未成熟细胞上：一种由抗体SG134鉴定的130至140 Kd的糖蛋白，以及一种由抗体MoU48识别的160至170 Kd的糖蛋白。
