Wheeler A W, Hickman B E, Fox B
Bencard Allergy Unit, Beecham Pharmaceuticals, Epsom, Surrey, U.K.
Mol Immunol. 1990 Jul;27(7):631-6. doi: 10.1016/0161-5890(90)90005-k.
Studies were carried out in order to confirm and extend knowledge of the physico-chemical properties of an allergenic material found in the pollen of the olive tree (Olea europea). The sera from 88% of patients who were sensitive to olive pollen contained IgE that reacted with a 19,000 Mr component and many also reacted to a 17,000 Mr band as shown by SDS-PAGE immunoblotting. A monoclonal antibody (OL-1) produced to the 19,000 Mr component also reacted with the 17,000 Mr band, and with bands at 21,000 and 41,000 under non-reduced conditions. HPLC separation followed by SDS-PAGE and immunoblotting of the fractions indicated that the allergen fraction from which the 19,000 and 17,000 components were derived had a mol. wt between 50 and 60 kD. Isoelectricfocusing followed by immunoblotting and development with (OL-1) indicated heterogeneity of the allergen with respect to pI values. Two of the strongest components of the six identified which reacted with (OL-1) had pIs of about 5.0 and 6.0 confirming the published data. The study therefore showed that olive pollen contains a number of allergenic components, with various mol. wts and pI values, with some epitopes in common, which may in the native state be bound together or aggregated.
开展了多项研究，以确认并拓展对橄榄树（油橄榄）花粉中一种变应原物质理化性质的认识。对橄榄花粉敏感的患者中，88%的血清含有与19,000道尔顿分子量成分发生反应的IgE，许多血清对17,000道尔顿分子量条带也有反应，这通过SDS - PAGE免疫印迹法得以显示。针对19,000道尔顿分子量成分产生的单克隆抗体（OL - 1），在非还原条件下也与17,000道尔顿分子量条带以及21,000和41,000道尔顿分子量的条带发生反应。通过HPLC分离，随后对各组分进行SDS - PAGE和免疫印迹分析表明，产生19,000和17,000道尔顿分子量成分的变应原组分分子量在50至60 kD之间。等电聚焦后进行免疫印迹并用（OL - 1）显色，结果表明变应原在pI值方面具有异质性。已鉴定出的与（OL - 1）发生反应的六种最强组分中，有两种的pI约为5.0和6.0，这证实了已发表的数据。因此，该研究表明橄榄花粉含有多种变应原成分，它们具有不同的分子量和pI值，有一些共同的表位，在天然状态下可能结合在一起或聚集。
