艾草和豚草花粉中常见致敏结构的鉴定。
Identification of common allergenic structures in mugwort and ragweed pollen.
作者信息
Hirschwehr R, Heppner C, Spitzauer S, Sperr W R, Valent P, Berger U, Horak F, Jäger S, Kraft D, Valenta R
机构信息
Institute of General and Experimental Pathology, AKH, University of Vienna, Austria.
出版信息
J Allergy Clin Immunol. 1998 Feb;101(2 Pt 1):196-206. doi: 10.1016/s0091-6749(98)70384-7.
UNLABELLED
Identification of common allergenic structures in mugwort and ragweed pollen.
BACKGROUND
Despite the rare occurrence of ragweed in Middle Europe, a surprisingly high number of patients allergic to mugwort, a frequently encountered weed, display IgE reactivity against ragweed pollen allergens.
OBJECTIVE
The aim of this study was to investigate whether the high prevalence of IgE reactivity against ragweed in patients allergic to mugwort is caused by the presence of common allergenic determinants. We also sought to characterize any cross-reactive allergens.
METHODS
Common allergenic structures in mugwort and ragweed pollen were characterized by qualitative IgE immunoblot inhibition experiments performed with natural allergen extracts and recombinant allergens. The degree of cross-reactivity was estimated by quantitative CAP-FEIA competitions. The clinical significance of cross-reactive IgE antibodies was studied with histamine release experiments and nasal provocation tests.
RESULTS
Mugwort and ragweed RAST values were significantly correlated in a population of 82 Austrian patients allergic to mugwort. IgE antibodies cross-reacted with allergens of comparable molecular weight that were present in both extracts. By using recombinant birch profilin and specific antisera for IgE inhibition experiments, profilin was identified as one of the cross-reactive components in mugwort and ragweed pollen. Preincubation of sera from patients allergic to mugwort with mugwort extract inhibited IgE binding to ragweed pollen extract greater than 80%. Mugwort and ragweed pollen extract induced comparable histamine release and reduction of nasal air flow in a patient with IgE reactivity against the major mugwort allergen Art v 1.
CONCLUSION
In addition to profilin, mugwort and ragweed pollen contain a number of cross-reactive allergens, among them the major mugwort allergen Art v 1. Cross-reactive IgE antibodies can lead to clinically significant allergic reactions.
未标记
艾蒿和豚草花粉中常见变应原结构的鉴定。
背景
尽管豚草在中欧罕见，但令人惊讶的是，大量对常见杂草艾蒿过敏的患者对豚草花粉过敏原表现出出Ig IgE 反应性。
目的
本研究旨在调查对艾蒿过敏的患者中针对豚草的 IgE 反应性高患病率是否由共同变应原决定簇的存在引起。我们还试图鉴定任何交叉反应性过敏原。
方法
通过用天然过敏原提取物和重组过敏原进行定性 IgE 免疫印迹抑制实验来鉴定艾蒿和豚草花粉中的常见变应原结构。通过定量 CAP-FEIA 竞争估计交叉反应程度。用组胺释放实验和鼻激发试验研究交叉反应性 IgE 抗体的临床意义。
结果
在 82 名对艾蒿过敏的奥地利患者群体中，艾蒿和豚草的 RAST 值显著相关。IgE 抗体与两种提取物中存在的分子量相当的过敏原发生交叉反应。通过使用重组桦树肌动蛋白结合蛋白和特异性抗血清进行 IgE 抑制实验，肌动蛋白结合蛋白被鉴定为艾蒿和豚草花粉中的交叉反应成分之一。用艾蒿提取物预孵育对艾蒿过敏患者的血清可使 IgE 与豚草花粉提取物的结合抑制超过 80%。艾蒿和豚草花粉提取物在一名对主要艾蒿过敏原 Art v 1 具有 IgE 反应性的患者中诱导了相当的组胺释放和鼻气流减少。
结论
除了肌动蛋白结合蛋白外，艾蒿和豚草花粉还含有许多交叉反应性过敏原，其中包括主要艾蒿过敏原 Art v 1。交叉反应性 IgE 抗体可导致具有临床意义的过敏反应。
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