引发酶识别的引发信号的结构特征：噬菌体G4互补DNA链合成起始位点的突变分析
Structural features of the priming signal recognized by primase: mutational analysis of the phage G4 origin of complementary DNA strand synthesis.
作者信息
Hiasa H, Sakai H, Komano T, Godson G N
机构信息
Department of Agricultural Chemistry, Kyoto University, Japan.
出版信息
Nucleic Acids Res. 1990 Aug 25;18(16):4825-31. doi: 10.1093/nar/18.16.4825.
45 mutations (insertion, deletion and base substitution) of the G4 Goric were tested for their functional activity in M13 and R199 in vivo. The critical mutants were also assayed for their ability to synthesize pRNA in vitro using SSB and primase. The results demonstrate that the secondary structure and spacing of stem-loops I and III are essential for Goric activity and that the 5'-CTG-3' sequence flanking stem-loop I is essential for initiation of pRNA synthesis.
对G4 Goric的45种突变（插入、缺失和碱基替换）在体内的M13和R199中进行了功能活性测试。还使用单链结合蛋白（SSB）和引发酶对关键突变体在体外合成pRNA的能力进行了测定。结果表明，茎环I和III的二级结构及间距对Goric活性至关重要，且茎环I侧翼的5'-CTG-3'序列对pRNA合成的起始至关重要。
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