Dehou M F, Schots R, Lacor P, Arras N, Verhavert P, Klöppel G, van Camp B
Department of Pathology, Academic Hospital of the Free University, Brussels, (VUB), Belgium.
Am J Clin Pathol. 1990 Sep;94(3):287-91. doi: 10.1093/ajcp/94.3.287.
Routinely processed bone marrow biopsies of 59 patients with untreated multiple myeloma (MM) and of 41 patients with monoclonal gammopathies of undetermined significance (MGUS) were immunocytochemically studied with the MB2 monoclonal antibody. In 54 of 59 biopsies of patients with MM, most neoplastic plasma cells showed strong cytoplasmic positivity to MB2. In contrast, only three biopsies of patients with MGUS contained highly MB2-positive plasma cells, whereas the plasma cells in the remaining biopsies were either negative (18 of 41) or revealed a weak dot-like staining of the cytoplasm (20 of 41). Plasma cells in tonsillar tissue, gastric and duodenal mucosae, and bone marrow with reactive plasmacytosis were not stained with MB2. These findings suggest that MB2 helps to distinguish between MM and MGUS. Because the five MB2-negative patients with MM were all in stage III and had very short survival time, neoplastic plasma cell staining with MB2 could also have a prognostic significance.
对59例未经治疗的多发性骨髓瘤（MM）患者和41例意义未明的单克隆丙种球蛋白病（MGUS）患者常规处理的骨髓活检标本，采用MB2单克隆抗体进行免疫细胞化学研究。在59例MM患者的活检标本中，有54例的大多数肿瘤性浆细胞对MB2呈强细胞质阳性。相比之下，MGUS患者中只有3例活检标本含有高度MB2阳性的浆细胞，其余活检标本中的浆细胞要么为阴性（41例中的18例），要么细胞质呈弱点状染色（41例中的20例）。扁桃体组织、胃和十二指肠黏膜以及有反应性浆细胞增多的骨髓中的浆细胞未被MB2染色。这些发现表明，MB2有助于区分MM和MGUS。由于5例MB2阴性的MM患者均处于III期且生存时间很短，肿瘤性浆细胞MB2染色也可能具有预后意义。
