Lokhorst H M, Boom S E, Terpstra W, Roholl P, Gerdes J, Bast B J
Department of Clinical Immunology, University Hospital, Utrecht, The Netherlands.
Br J Haematol. 1988 Aug;69(4):477-81. doi: 10.1111/j.1365-2141.1988.tb02402.x.
The monoclonal antibody Ki-67 reacts with a nuclear antigen that is present only in proliferating cells. The proportion of Ki-67 positive cells may therefore serve as a reliable measurement for the growth fraction in normal and neoplasmic cell populations. We have tested the significance of the MoAb Ki-67 in the classification of monoclonal gammopathy and compared the results with the plasma cell labelling index. In benign monoclonal gammopathy the percentage of Ki-67 positive plasma cells (median 1.6%) was significantly lower than in untreated multiple myeloma (median 9.6). Among the patients with more than 10% Ki-67 positive plasma cells there were some very short survivors. The largest growth fractions (median 41.8%) were found in patients with relapsing multiple myeloma indicating here a different growth pattern more resembling the high-grade lymphomas. A linear correlation between the proportion of Ki-67 positive plasma cells and the labelling index was not found. Determination of the plasma cell growth fraction with the monoclonal antibody Ki-67 in monoclonal gammopathy may help to discriminate benign monoclonal gammopathy from multiple myeloma and will probably identify a subgroup of multiple myeloma patients with a poor prognosis, including those with relapsing multiple myeloma.
单克隆抗体Ki-67与一种仅存在于增殖细胞中的核抗原发生反应。因此，Ki-67阳性细胞的比例可作为正常和肿瘤细胞群体中生长分数的可靠测量指标。我们测试了单克隆抗体Ki-67在单克隆丙种球蛋白病分类中的意义，并将结果与浆细胞标记指数进行了比较。在良性单克隆丙种球蛋白病中，Ki-67阳性浆细胞的百分比（中位数为1.6%）显著低于未经治疗的多发性骨髓瘤（中位数为9.6%）。在Ki-67阳性浆细胞超过10%的患者中，有一些存活时间非常短。复发的多发性骨髓瘤患者中发现了最大的生长分数（中位数为41.8%），这表明此处存在一种不同的生长模式，更类似于高级别淋巴瘤。未发现Ki-67阳性浆细胞比例与标记指数之间存在线性相关性。用单克隆抗体Ki-67测定单克隆丙种球蛋白病中的浆细胞生长分数可能有助于区分良性单克隆丙种球蛋白病和多发性骨髓瘤，并且可能识别出预后不良的多发性骨髓瘤患者亚组，包括复发的多发性骨髓瘤患者。
