Dyubko Tatyana S, Onishchenko Elena V, Pivovarenko Vasyl G
Institute for Problems of Cryobiology and Cryomedicine, Ukrainian National Academy of Sciences, 23 Pereyaslavskaya Str., Kharkov, 61015, Ukraine.
J Fluoresc. 2006 Nov;16(6):817-23. doi: 10.1007/s10895-006-0089-5. Epub 2006 Sep 15.
The influence of low molecular weight cryoprotectants (CPs) such as glycerol (GL), 1,2-propanediol (PD) and dimethylsulfoxide (DMSO) on the structure of rat liver microsomal membranes on the stages of equilibration and upon freezing up to -196 degrees C was studied using a multiparametric fluorescent probe of flavonol nature. It was estimated that the studied CPs have individual concentration ranges defining low amplitude of their action on biomembranes. An exceeding of these ranges strongly increases the violation of membrane native structure already at the stage of incubation with CPs, strengthening it during the freezing procedure. According to the perturbation effect on microsomal membranes the studied CPs can be arranged in a sequence: DMSO>PD>GL.
使用黄酮醇类多参数荧光探针，研究了低分子量冷冻保护剂（CPs），如甘油（GL）、1,2 - 丙二醇（PD）和二甲基亚砜（DMSO）在平衡阶段以及冷冻至 -196℃时对大鼠肝脏微粒体膜结构的影响。据估计，所研究的CPs具有各自的浓度范围，在此范围内它们对生物膜的作用幅度较小。超过这些范围会强烈增加在与CPs孵育阶段对膜天然结构的破坏，并在冷冻过程中进一步加剧这种破坏。根据对微粒体膜的扰动作用，所研究的CPs可按以下顺序排列：DMSO>PD>GL。
