Matsuo Junji
Division of Medical Technology, Department of Health Sciences, Hokkaido University School of Medicine, North-12, West-5, Kita-ku, Sapporo 060-0812, Japan.
Parasitol Res. 2007 Sep;101(4):907-11. doi: 10.1007/s00436-007-0559-y. Epub 2007 May 13.
A pellet freezing method, one type of rapid freezing protocol, was established for Trichomonas vaginalis. As cryoprotectants, 5-20% dimethylsulfoxide (DMSO), ethylene glycol, propylene glycol, and glycerol were used. Of these, DMSO showed a high protective effect, with a maximum effect at a concentration of 20%. No effect of equilibration time was recognized with any of the cryoprotectants. Therefore, the optimal condition was deemed 20% DMSO without equilibration. The pellet freezing method was also compared with the conventional slow cooling protocol. The survival rate was 73 +/- 8% with the slow cooling protocol and 48 +/- 4% with the pellet freezing method. The delay in growth with the pellet freezing method was no more than 5.4 h compared with the slow cooling protocol, suggesting its validity as a technique for cryopreservation of T. vaginalis.
已为阴道毛滴虫建立了一种颗粒冷冻方法，这是一种快速冷冻方案。作为冷冻保护剂，使用了5%-20%的二甲基亚砜（DMSO）、乙二醇、丙二醇和甘油。其中，DMSO显示出较高的保护作用，在浓度为20%时效果最佳。使用任何一种冷冻保护剂均未发现平衡时间有影响。因此，最佳条件被认为是20% DMSO且无需平衡。还将颗粒冷冻方法与传统的缓慢冷却方案进行了比较。缓慢冷却方案的存活率为73±8%，颗粒冷冻方法的存活率为48±4%。与缓慢冷却方案相比，颗粒冷冻方法导致的生长延迟不超过5.4小时，表明其作为阴道毛滴虫冷冻保存技术的有效性。
