Tamplin M L, Jalali R, Ahmed M K, Colwell R R
Center of Marine Biotechnology, Maryland Biotechnology Institute, University of Maryland, Baltimore 21202.
Can J Microbiol. 1990 Jun;36(6):409-13. doi: 10.1139/m90-071.
Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which corresponded with epitypes described previously for V. cholerae O1 classical biotype CT-B (CT1), El Tor biotype CT-B (CT2), and a unique V. cholerae non-O1 CT-B (CT3), which lacked an epitope located in or near the GM1 ganglioside binding site of 569B CT-B. Vibrio mimicus CT-B was immunologically indistinguishable from 569B CT-B. These and previous results define six epitopes on 569B CT-B, and a fourth epitope in or near the GM1 ganglioside binding site.
用与霍乱弧菌O1菌株569B霍乱毒素（CT-B）的B亚基发生反应的单克隆抗体来鉴定霍乱弧菌非O1型菌株和拟态弧菌CT-B的独特表位和共有表位。霍乱弧菌非O1型菌株产生的CT-B呈现出三种单克隆抗体反应模式（表型），这与先前描述的霍乱弧菌O1型古典生物型CT-B（CT1）、埃尔托生物型CT-B（CT2）以及一种独特的霍乱弧菌非O1型CT-B（CT3）的表型相对应，CT3缺乏位于569B CT-B的GM1神经节苷脂结合位点内或其附近的一个表位。拟态弧菌CT-B在免疫学上与569B CT-B无法区分。这些结果以及之前的结果确定了569B CT-B上的六个表位，以及GM1神经节苷脂结合位点内或其附近的第四个表位。
