Yamamoto Masahiro, Arai Hiroyuki, Ishii Masaharu, Igarashi Yasuo
Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
FEMS Microbiol Lett. 2006 Oct;263(2):189-93. doi: 10.1111/j.1574-6968.2006.00415.x.
Hydrogenobacter thermophilus TK-6 is a thermophilic, hydrogen-oxidizing bacterium that fixes carbon dioxide as a sole carbon source via the reductive tricarboxylic acid cycle. 2-Oxoglutarate:ferredoxin oxidoreductase (OGOR) is one of the key enzymes in the pathway. Strain TK-6 has at least two isozymes of OGOR, namely For and Kor. These OGORs showed different expression patterns under aerobic conditions than under anaerobic conditions. In this work, we developed a homologous recombination method for Hydrogenobacter, and constructed a For mutant and a Kor mutant. Observation of phenotypes of the mutant strains showed that Kor was essential for anaerobic growth and that For activity supported robust aerobic growth of the organism.
嗜热氢杆菌TK-6是一种嗜热的、氧化氢的细菌，它通过还原性三羧酸循环将二氧化碳固定为唯一碳源。2-酮戊二酸：铁氧化还原蛋白氧化还原酶（OGOR）是该途径中的关键酶之一。TK-6菌株至少有两种OGOR同工酶，即For和Kor。这些OGOR在有氧条件下与厌氧条件下表现出不同的表达模式。在这项工作中，我们开发了一种嗜热氢杆菌的同源重组方法，并构建了一个For突变体和一个Kor突变体。对突变菌株表型观察表明，Kor对厌氧生长至关重要且For活性支持该生物体的强劲有氧生长。
