Toro Ana Flávia, Malta Marília Brinati, Soares Sabrina Lucio, Da Rocha Guilherme Casoni, da Silva Lira Marcela, De Oliveira Thais Abbate, Takehara Harumi Ando, Lopes-Ferreira Mônica, Santoro Marcelo Larami, Guidolin Rosalvo, Gondo Higashi Hisako, Fernandes Irene, Barbaro Katia Cristina
Laboratory of Immunopathology, Butantan Institute, Av. Vital Brazil 1500, 05503-900 São Paulo, SP, Brazil.
Toxicon. 2006 Nov;48(6):649-61. doi: 10.1016/j.toxicon.2006.07.029. Epub 2006 Jul 25.
The ability of IgG(T) and IgGa subclasses--isolated by liquid chromatography from equine arachnidic antivenom (AAV)-to neutralize toxic activities of Loxosceles gaucho, Phoneutria nigriventer and Tityus serrulatus venoms as well as to remove venom toxins from circulation was investigated. These subclasses showed similar antibody titers against L. gaucho, P. nigriventer and T. serrulatus venoms, and by immunoblotting few differences were observed in the recognition pattern of venom antigens. IgG(T) and IgGa neutralized 100% lethality induced by L. gaucho and 50% of P. nigriventer venom, but IgGa failed to neutralize T. serrulatus venom, in contrast to IgG(T). Both subclasses neutralized local reactions and dermonecrosis induced by L. gaucho venom in rabbits. In mice, IgG(T) and IgGa partially neutralized the edematogenic activity induced by P. nigriventer and T. serrulatus venoms, but only IgG(T) neutralized (ca. 81%) the nociceptive activity induced by T. serrulatus venom. Both subclasses failed to neutralize nociceptive activity induced by P. nigriventer venom. IgG(T) reduced the serum venom levels of animals injected with L. gaucho, P. nigriventer or T. serrulatus venoms, while IgGa solely reduced L. gaucho and P. nigriventer venoms levels. Our results demostrate that IgG(T) and IgGa subclasses neutralize toxic activities induced by P. nigriventer, T. serrulatus and L. gaucho venoms with different efficacies, as well as depurate these venoms from circulation.
研究了通过液相色谱法从马蛛毒抗蛇毒血清（AAV）中分离出的IgG(T)和IgGa亚类中和巴西游走蛛、黑腹捕鸟蛛和锯齿蝎毒液的毒性活性以及从循环中清除毒液毒素的能力。这些亚类对巴西游走蛛、黑腹捕鸟蛛和锯齿蝎毒液显示出相似的抗体效价，并且通过免疫印迹法在毒液抗原的识别模式上观察到很少的差异。IgG(T)和IgGa中和了巴西游走蛛毒液诱导的100%致死率以及黑腹捕鸟蛛毒液的50%，但与IgG(T)相反，IgGa未能中和锯齿蝎毒液。这两个亚类都中和了巴西游走蛛毒液在兔子中诱导的局部反应和皮肤坏死。在小鼠中，IgG(T)和IgGa部分中和了黑腹捕鸟蛛和锯齿蝎毒液诱导的水肿活性，但只有IgG(T)中和了（约81%）锯齿蝎毒液诱导的伤害感受活性。这两个亚类都未能中和黑腹捕鸟蛛毒液诱导的伤害感受活性。IgG(T)降低了注射巴西游走蛛、黑腹捕鸟蛛或锯齿蝎毒液的动物的血清毒液水平，而IgGa仅降低了巴西游走蛛和黑腹捕鸟蛛毒液的水平。我们的结果表明，IgG(T)和IgGa亚类以不同的效力中和黑腹捕鸟蛛、锯齿蝎和巴西游走蛛毒液诱导的毒性活性，并从循环中清除这些毒液。
