Cheng S H, Espino P C, Marshall J, Harvey R, Smith A E
Laboratory of Cellular Regulation, Genzyme Corporation, Framingham, Massachusetts 01701.
Mol Cell Biol. 1990 Oct;10(10):5569-74. doi: 10.1128/mcb.10.10.5569-5574.1990.
Our results indicate that only one type of tyrosine kinase is present within each middle-T antigen-tyrosine kinase complex, suggesting that middle-T antigen forms separate complexes with different tyrosine kinases. Furthermore, we determined that there is only one molecule of middle-T antigen within any one of these complexes. We interpret this to mean that in any given cell, polyomavirus transformation involves, at least in part, the simultaneous deregulation of a number of separate pathways controlling cellular proliferation. Finally, we also demonstrate that the separate middle-T:pp60c-src and middle-T:pp59c-fyn complexes are each able to interact with the same cellular p81/85-kDa phosphoprotein, a possible component of the phosphatidylinositol kinase.
我们的结果表明，每个中T抗原 - 酪氨酸激酶复合物中仅存在一种类型的酪氨酸激酶，这表明中T抗原与不同的酪氨酸激酶形成单独的复合物。此外，我们确定在这些复合物中的任何一个中仅存在一个中T抗原分子。我们将此解释为，在任何给定细胞中，多瘤病毒转化至少部分涉及同时解除对许多控制细胞增殖的独立途径的调节。最后，我们还证明，单独的中T：pp60c-src和中T：pp59c-fyn复合物各自能够与相同的细胞p81/85-kDa磷蛋白相互作用，该磷蛋白可能是磷脂酰肌醇激酶的一个组分。
