• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

针对劳氏肉瘤病毒pp60src的单克隆抗体可与源自禽类和哺乳动物的具有酶活性的细胞pp60src发生反应。

Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin.

作者信息

Parsons S J, McCarley D J, Ely C M, Benjamin D C, Parsons J T

出版信息

J Virol. 1984 Aug;51(2):272-82. doi: 10.1128/JVI.51.2.272-282.1984.

DOI:10.1128/JVI.51.2.272-282.1984
PMID:6205164
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254436/
Abstract

The derivation and characterization of 22 hybridoma clones producing monoclonal antibodies (Mabs) specific for the transforming protein of Rous sarcoma virus, pp60src, are described. All Mabs reacted with pp60v-src encoded by Prague, Schmidt-Ruppin, and Bratislava 77 strains of Rous sarcoma virus. Of these Mabs, 10 efficiently immunoprecipitated pp60c-src from chicken embryo cells. Of these 10 Mabs, 2 (GD11 and EB8) readily detected pp60c-src from a variety of rodent and human cultured cells and from rat brain tissue in an in vitro immune complex kinase assay. Mapping experiments have tentatively localized the determinant(s) recognized by GD11 and EB8 to a region of the src protein bounded by amino acid residues 82 to 169, whereas the remaining Mabs appeared to recognize determinants residing within residues 1 to 82 or 169 to 173. Most of the Mabs complexed denatured pp60v-src in a Western immunoblot, and several were used to localize pp60v-src in Rous sarcoma virus-transformed chicken embryo cells by indirect immunofluorescence microscopy.

摘要

本文描述了22个杂交瘤克隆的衍生和特性，这些克隆产生针对劳氏肉瘤病毒转化蛋白pp60src的单克隆抗体（Mabs）。所有单克隆抗体都与由布拉格、施密特-鲁平及布拉迪斯拉发77株劳氏肉瘤病毒编码的pp60v-src发生反应。在这些单克隆抗体中，有10个能有效地从鸡胚细胞中免疫沉淀pp60c-src。在这10个单克隆抗体中，有2个（GD11和EB8）在体外免疫复合物激酶分析中能轻易地从多种啮齿动物和人类培养细胞以及大鼠脑组织中检测到pp60c-src。定位实验初步将GD11和EB8识别的决定簇定位到src蛋白中由氨基酸残基82至169界定的区域，而其余单克隆抗体似乎识别位于残基1至82或169至173内的决定簇。大多数单克隆抗体在蛋白质免疫印迹中与变性的pp60v-src形成复合物，并且有几种被用于通过间接免疫荧光显微镜在劳氏肉瘤病毒转化的鸡胚细胞中定位pp60v-src。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/47476a301b5e/jvirol00131-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/3cb7686b7eb4/jvirol00131-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/66d29d5f7da4/jvirol00131-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/1db9513f5ff1/jvirol00131-0017-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/1ad72237756c/jvirol00131-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/67b984d704d4/jvirol00131-0018-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/8a7329c8d2d5/jvirol00131-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/fe63c0a1da45/jvirol00131-0020-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/0e58b7bd785c/jvirol00131-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/792aa54c1a54/jvirol00131-0021-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/47476a301b5e/jvirol00131-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/3cb7686b7eb4/jvirol00131-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/66d29d5f7da4/jvirol00131-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/1db9513f5ff1/jvirol00131-0017-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/1ad72237756c/jvirol00131-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/67b984d704d4/jvirol00131-0018-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/8a7329c8d2d5/jvirol00131-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/fe63c0a1da45/jvirol00131-0020-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/0e58b7bd785c/jvirol00131-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/792aa54c1a54/jvirol00131-0021-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75dc/254436/47476a301b5e/jvirol00131-0022-a.jpg

相似文献

1
Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin.针对劳氏肉瘤病毒pp60src的单克隆抗体可与源自禽类和哺乳动物的具有酶活性的细胞pp60src发生反应。
J Virol. 1984 Aug;51(2):272-82. doi: 10.1128/JVI.51.2.272-282.1984.
2
Monoclonal antibody against the carboxy terminal peptide of pp60src of Rous sarcoma virus reacts with native pp60src.针对劳氏肉瘤病毒pp60src羧基末端肽的单克隆抗体与天然pp60src发生反应。
EMBO J. 1982;1(12):1479-85. doi: 10.1002/j.1460-2075.1982.tb01343.x.
3
Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.识别禽肉瘤病毒转化蛋白的单克隆抗体的分离
J Virol. 1983 Nov;48(2):352-60. doi: 10.1128/JVI.48.2.352-360.1983.
4
Isolation and partial characterization of a monoclonal antibody to the Rous sarcoma virus transforming protein pp60src.劳斯肉瘤病毒转化蛋白pp60src单克隆抗体的分离与部分特性鉴定
J Virol. 1983 Mar;45(3):1190-4. doi: 10.1128/JVI.45.3.1190-1194.1983.
5
pp60v-src tyrosine kinase is expressed and active in sarcoma-free avian embryos microinjected with Rous sarcoma virus.pp60v-src酪氨酸激酶在注射了劳氏肉瘤病毒的无肉瘤禽胚胎中表达并具有活性。
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7587-91. doi: 10.1073/pnas.85.20.7587.
6
Tyrosine phosphorylation of a 50K cellular polypeptide associated with the Rous sarcoma virus transforming protein pp60src.与劳氏肉瘤病毒转化蛋白pp60src相关的一种50K细胞多肽的酪氨酸磷酸化作用。
Mol Cell Biol. 1982 Feb;2(2):199-206. doi: 10.1128/mcb.2.2.199-206.1982.
7
Rous sarcoma virus variants that encode src proteins with an altered carboxy terminus are defective for cellular transformation.编码具有改变的羧基末端的src蛋白的劳氏肉瘤病毒变体在细胞转化方面存在缺陷。
J Virol. 1985 Aug;55(2):314-21. doi: 10.1128/JVI.55.2.314-321.1985.
8
Amino acid alterations within a highly conserved region of the Rous sarcoma virus src gene product pp60src inactivate tyrosine protein kinase activity.劳氏肉瘤病毒src基因产物pp60src高度保守区域内的氨基酸改变会使酪氨酸蛋白激酶活性失活。
Mol Cell Biol. 1984 May;4(5):862-6. doi: 10.1128/mcb.4.5.862-866.1984.
9
Size-variant pp60src proteins of recovered avian sarcoma viruses interact with adhesion plaques as peripheral membrane proteins: effects on cell transformation.恢复的禽肉瘤病毒的大小可变的pp60src蛋白作为外周膜蛋白与粘着斑相互作用：对细胞转化的影响。
Mol Cell Biol. 1984 Mar;4(3):454-67. doi: 10.1128/mcb.4.3.454-467.1984.
10
Highly specific antibody to Rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules.针对劳氏肉瘤病毒src基因产物的高度特异性抗体识别出一种新的pp60v-src和pp60c-src分子群体。
J Cell Biol. 1985 Feb;100(2):409-17. doi: 10.1083/jcb.100.2.409.

引用本文的文献

1
CD3ζ-Mediated Signaling Protects Retinal Ganglion Cells in Glutamate Excitotoxicity of the Retina.CD3ζ 介导的信号转导在视网膜谷氨酸兴奋性毒性中保护视网膜神经节细胞。
Cells. 2024 Jun 8;13(12):1006. doi: 10.3390/cells13121006.
2
Epidermal growth factor receptor translocation to the mitochondria: regulation and effect.表皮生长因子受体转位至线粒体：调控与效应。
J Biol Chem. 2009 Dec 25;284(52):36592-36604. doi: 10.1074/jbc.M109.000760. Epub 2009 Oct 19.
3
c-Src tyrosine kinase, a critical component for 5-HT2A receptor-mediated contraction in rat aorta.

本文引用的文献

1
One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography.通过DEAE亲和蓝胶色谱法从腹水原液中一步纯化小鼠单克隆抗体。
J Immunol Methods. 1982 Sep 30;53(3):313-9. doi: 10.1016/0022-1759(82)90178-8.
2
Masking of extracellular collagen and the co-distribution of collagen and fibronectin during matrix formation by cultured embryonic fibroblasts.培养的胚胎成纤维细胞在基质形成过程中细胞外胶原蛋白的掩盖以及胶原蛋白与纤连蛋白的共分布
J Cell Sci. 1982 Jun;55:35-50. doi: 10.1242/jcs.55.1.35.
3
Synthetic peptide fragment of src gene product inhibits the src protein kinase and crossreacts immunologically with avian onc kinases and cellular phosphoproteins.
c-Src酪氨酸激酶，是大鼠主动脉中5-HT2A受体介导收缩的关键组成部分。
J Physiol. 2008 Aug 15;586(16):3855-69. doi: 10.1113/jphysiol.2008.153593. Epub 2008 Jul 3.
4
Complex formation with focal adhesion kinase: A mechanism to regulate activity and subcellular localization of Src kinases.与粘着斑激酶形成复合物：一种调节Src激酶活性和亚细胞定位的机制。
Mol Biol Cell. 1999 Oct;10(10):3489-505. doi: 10.1091/mbc.10.10.3489.
5
Rous sarcoma virus direct repeat cis elements exert effects at several points in the virus life cycle.劳氏肉瘤病毒直接重复顺式元件在病毒生命周期的多个环节发挥作用。
J Virol. 1997 Dec;71(12):9150-6. doi: 10.1128/JVI.71.12.9150-9156.1997.
6
The integrity of the SH3 binding motif of AFAP-110 is required to facilitate tyrosine phosphorylation by, and stable complex formation with, Src.AFAP-110的SH3结合基序的完整性是促进Src介导的酪氨酸磷酸化以及与Src形成稳定复合物所必需的。
Mol Cell Biochem. 1997 Oct;175(1-2):243-52. doi: 10.1023/a:1006840104666.
7
Phosphotyrosine phosphatase activity associated with c-Src in large multimeric complexes isolated from adrenal medullary chromaffin cells.从肾上腺髓质嗜铬细胞分离出的大型多聚体复合物中，与c-Src相关的磷酸酪氨酸磷酸酶活性。
Biochem J. 1997 Aug 15;326 ( Pt 1)(Pt 1):271-7. doi: 10.1042/bj3260271.
8
Polyomavirus middle-T antigen associates with the kinase domain of Src-related tyrosine kinases.多瘤病毒中T抗原与Src相关酪氨酸激酶的激酶结构域相关联。
J Virol. 1996 Mar;70(3):1323-30. doi: 10.1128/JVI.70.3.1323-1330.1996.
9
Palmitylation of an amino-terminal cysteine motif of protein tyrosine kinases p56lck and p59fyn mediates interaction with glycosyl-phosphatidylinositol-anchored proteins.蛋白酪氨酸激酶p56lck和p59fyn氨基末端半胱氨酸基序的棕榈酰化介导了与糖基磷脂酰肌醇锚定蛋白的相互作用。
Mol Cell Biol. 1993 Oct;13(10):6385-92. doi: 10.1128/mcb.13.10.6385-6392.1993.
10
Identification of sequences required for the efficient localization of the focal adhesion kinase, pp125FAK, to cellular focal adhesions.鉴定使粘着斑激酶pp125FAK有效定位于细胞粘着斑所需的序列。
J Cell Biol. 1993 Nov;123(4):993-1005. doi: 10.1083/jcb.123.4.993.
src基因产物的合成肽片段可抑制src蛋白激酶，并与禽肿瘤激酶和细胞磷蛋白发生免疫交叉反应。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7412-6. doi: 10.1073/pnas.78.12.7412.
4
Antibodies to a defined region of pp60src neutralize the tyrosine-specific kinase activity.针对pp60src特定区域的抗体可中和酪氨酸特异性激酶活性。
J Biol Chem. 1983 Sep 25;258(18):11219-28.
5
Cellular oncogenes and retroviruses.细胞癌基因与逆转录病毒。
Annu Rev Biochem. 1983;52:301-54. doi: 10.1146/annurev.bi.52.070183.001505.
6
Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.识别禽肉瘤病毒转化蛋白的单克隆抗体的分离
J Virol. 1983 Nov;48(2):352-60. doi: 10.1128/JVI.48.2.352-360.1983.
7
Polyoma virus transforming protein associates with the product of the c-src cellular gene.多瘤病毒转化蛋白与c-src细胞基因的产物相关联。
Nature. 1983;303(5916):435-9. doi: 10.1038/303435a0.
8
Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus.与劳氏肉瘤病毒src基因同源的细胞基因的结构与序列以及产生转化病毒的机制。
Cell. 1983 Mar;32(3):881-90. doi: 10.1016/0092-8674(83)90073-9.
9
Nucleotide sequence of Rous sarcoma virus.劳氏肉瘤病毒的核苷酸序列。
Cell. 1983 Mar;32(3):853-69. doi: 10.1016/0092-8674(83)90071-5.
10
Site-directed mutagenesis of the src gene of Rous sarcoma virus: construction and characterization of a deletion mutant temperature sensitive for transformation.劳氏肉瘤病毒src基因的定点诱变：对转化温度敏感的缺失突变体的构建与特性分析
J Virol. 1982 Nov;44(2):683-91. doi: 10.1128/JVI.44.2.683-691.1982.