Matsumoto S, Hanai T, Matsuura T, Uemura H, Nishioka T, Akiyama T
Department of Urology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.
Transplant Proc. 2006 Sep;38(7):2014-5. doi: 10.1016/j.transproceed.2006.06.015.
8-Hydroxy-2'-deoxyguanosine (8-OHdG) is an oxidant of deoxyguanosine, a base in the construction of DNA. We examined the extent of DNA damage to the graft through oxidative stress after renal transplantation. Seven renal transplant patients were enrolled in this study. Before reperfusion of the grafts, the level of serum 8-OHdG was measured using enzyme-linked immunosorbent assay. No relationship was found between the level of serum 8-OHdG just before reperfusion and the postoperative course. In all cases, the level of serum 8-OHdG increased after reperfusion and decreased within 2 hours. In six cases, it remained almost the same as the preoperative level. A faster rate of decrease from the first peak of serum 8-OHdG was associated with a lower nadir serum creatinine and reduced occurrence of acute rejection. This study suggested that immediate calming of the oxidative stress following reperfusion may potentially have a positive influence on graft prognosis. In addition, biomarkers of oxidative stress may predict graft prognosis.
8-羟基-2'-脱氧鸟苷（8-OHdG）是脱氧鸟苷的一种氧化产物，脱氧鸟苷是DNA结构中的一种碱基。我们研究了肾移植后氧化应激对移植肾DNA损伤的程度。本研究纳入了7例肾移植患者。在移植肾再灌注前，采用酶联免疫吸附测定法测定血清8-OHdG水平。再灌注前血清8-OHdG水平与术后病程之间未发现相关性。在所有病例中，血清8-OHdG水平在再灌注后升高，并在2小时内下降。在6例病例中，其水平几乎与术前水平相同。血清8-OHdG第一个峰值下降速度较快与较低的血清肌酐最低点及急性排斥反应发生率降低相关。本研究提示，再灌注后立即缓解氧化应激可能对移植肾预后产生积极影响。此外，氧化应激生物标志物可能预测移植肾预后。
