Detection of T1-oligonucleotides of the foot-and-mouth disease virus RNA [correction of DNA] by silver staining.

Viral (v) RNA was isolated from purified foot-and-mouth disease virus (FMDV) by phenol-chloroform-isoamylalcohol treatment, digested by RNase T1 and separated by one-dimensional polyacrylamide gel electrophoresis (PAGE). The oligonucleotides were detected by silver staining. About 45 micrograms of vRNA corresponding to about 100 ml of infectious BHK-21 cell culture fluid yielded a pattern of nearly 20 bands sufficient to differentiate between the FMD viruses.