Jamieson S, Rudland P S
Cancer and Polio Research Fund Laboratories, Liverpool University, United Kingdom.
Am J Pathol. 1990 Sep;137(3):629-41.
The rat mammary 37 epithelial cell line yields non-metastasizing adenomas in syngeneic rats. On cellular DNA transfection, a series of cell lines have been isolated that grow in drug-selective medium. Representative transfected cell lines all yield tumors in rats that consist predominantly of spindle cells, but two also contain epithelial-like cells and glandlike elements (C18P, C19P). Immunocytochemical staining for milk fat globule membrane antigens, human callus keratin, and laminin confirms the identity of the epithelial cells and suggests a (myo)epithelial origin for the spindle cells. Some of the transfected cell lines also generate well-differentiated metaplastic elements in their tumors. One cell line (CT4-41) produces rhabdomyoblastic and possibly smooth-muscle-related elements; two (C18P, C19P) produce squamous metaplasia and sebaceous elements; and two (CL1-31, C11P) produce cartilaginous elements. The identities of the heterologous elements are confirmed by immunocytochemical staining for myoglobin, actin (CT4-41), keratin (C18P, C19P), type II collagen, and type II keratan sulfate (CL1-31). Those cell lines that have acquired the ability to metastasize from subcutaneous sites (CT4-41, C18P) reproduce the same metaplastic elements in their metastases. Thus, a cloned mammary epithelial cell line can be made to generate many of the well-differentiated, heterologous elements observed in human breast carcinomas, and this change is often associated with the rat cells acquiring metastatic properties.
大鼠乳腺37上皮细胞系在同基因大鼠中产生非转移性腺瘤。通过细胞DNA转染，已分离出一系列能在药物选择培养基中生长的细胞系。代表性的转染细胞系在大鼠体内均产生肿瘤，这些肿瘤主要由梭形细胞组成，但其中两个细胞系还含有上皮样细胞和腺样成分（C18P、C19P）。对乳脂肪球膜抗原、人角质形成细胞角蛋白和层粘连蛋白进行免疫细胞化学染色，证实了上皮细胞的特性，并提示梭形细胞起源于（肌）上皮。一些转染细胞系在其肿瘤中还产生分化良好的化生成分。一个细胞系（CT4 - 41）产生横纹肌母细胞及可能与平滑肌相关的成分；两个细胞系（C18P、C19P）产生鳞状化生和皮脂腺成分；两个细胞系（CL1 - 31、C11P）产生软骨成分。通过对肌红蛋白、肌动蛋白（CT4 - 41）、角蛋白（C18P、C19P）、II型胶原蛋白和II型硫酸角质素（CL1 - 31）进行免疫细胞化学染色，证实了这些异源成分的特性。那些已获得从皮下部位转移能力的细胞系（CT4 - 41、C18P）在转移灶中重现了相同的化生成分。因此，一个克隆的乳腺上皮细胞系能够产生人类乳腺癌中观察到的许多分化良好的异源成分，并且这种变化通常与大鼠细胞获得转移特性相关。
