Sato Yumiko, Nakamura Naoya, Nakamura Satoko, Sakugawa Sumie, Ichimura Koichi, Tanaka Takehiro, Ohara Nobuya, Oka Takeshi, Kondo Eisaku, Yoshino Tadashi
Department of Pathology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
Mod Pathol. 2006 Dec;19(12):1578-84. doi: 10.1038/modpathol.3800692. Epub 2006 Sep 15.
It remains unclear whether or not diffuse large B-cell lymphomas of extranodal sites arise from mucosa-associated lymphoid tissue (MALT) lymphomas. We studied the clinicopathological features of MALT lymphoma and diffuse large B-cell lymphoma in the thyroid gland, with special reference to VH usage of immunoglobulin gene rearrangement, to clarify the relationships between these two types of lymphomas. In addition, t(11; 18) (q21; q21) translocation was examined by multiplex reverse transcription-polymerase chain reaction. We examined 58 patients with primary thyroid lymphoma: 31 (male seven and female 24) with MALT lymphoma and 27 (male three and female 24) with diffuse large B-cell lymphoma. Interestingly, the sequence of VH genes revealed that the two subtypes differed significantly in their use of the VH4 family (P < 0.05). Of the seven MALT lymphomas, three used the VH4 family and the other four used the VH3 family, whereas eight out of nine diffuse large B-cell lymphoma used the VH3 family, one used the VH1 family, and none used the VH4 family. It was also interesting that, in one diffuse large B-cell lymphoma patient with MALT lymphoma, the diffuse large B-cell lymphoma component used the VH3 family and the MALT lymphoma component used the VH4 family. These data imply that, in a subset of cases, these two subtypes do not share a common origin and that at least some diffuse large B-cell lymphomas have a de novo origin. No t(11; 18) (q21; q21) was detected in thyroid lymphomas, which are different from MALT lymphoma of the stomach, lungs, large intestine and ocular adnexa. This strongly indicated that the presence of t(11; 18) (q21; q21) in MALT lymphoma is organ-specific.
结外部位的弥漫性大B细胞淋巴瘤是否起源于黏膜相关淋巴组织（MALT）淋巴瘤仍不清楚。我们研究了甲状腺MALT淋巴瘤和弥漫性大B细胞淋巴瘤的临床病理特征，特别参考免疫球蛋白基因重排的VH使用情况，以阐明这两种淋巴瘤之间的关系。此外，通过多重逆转录-聚合酶链反应检测t(11; 18) (q21; q21)易位。我们检查了58例原发性甲状腺淋巴瘤患者：31例（男性7例，女性24例）为MALT淋巴瘤，27例（男性3例，女性24例）为弥漫性大B细胞淋巴瘤。有趣的是，VH基因序列显示这两种亚型在VH4家族的使用上有显著差异（P < 0.05）。在7例MALT淋巴瘤中，3例使用VH4家族，另外4例使用VH3家族，而9例弥漫性大B细胞淋巴瘤中有8例使用VH3家族，1例使用VH1家族，无1例使用VH4家族。同样有趣的是，在1例同时患有MALT淋巴瘤的弥漫性大B细胞淋巴瘤患者中，弥漫性大B细胞淋巴瘤成分使用VH3家族，而MALT淋巴瘤成分使用VH4家族。这些数据表明，在一部分病例中，这两种亚型并非起源相同，且至少部分弥漫性大B细胞淋巴瘤是新发起源。在甲状腺淋巴瘤中未检测到t(11; 18) (q21; q21)，这与胃、肺、大肠和眼附属器的MALT淋巴瘤不同。这有力地表明MALT淋巴瘤中t(11; 18) (q21; q21)的存在具有器官特异性。
