Korotkov E V
Izv Akad Nauk SSSR Biol. 1990 May-Jun(3):359-65.
A method of computer analysis of DNA sequences has been proposed. It is based on information similarity of compared sequences and it significantly increases the usefulness of the computer analysis. This approach has been applied to the search of interconnected areas of Alu-repeats and replication origins of p15A and R6K plasmids. An Alu-like region located in the first stem of the secondary structure of RNA-1 and E. coli RNA-polymerase binding site has been found in the p15A. On R6K replication origin, Alu-like repeats have been found in the area of tandem 22 bp repeats. This comparison also allowed to reveal hidden periodicity of the sequence of human Alu-repeat. A hypothesis that explained the data obtained has been proposed. The proposed approach may be used as a method for revealing DNA sequences that have similar genetic functions.
已经提出了一种DNA序列的计算机分析方法。它基于比较序列的信息相似性，显著提高了计算机分析的实用性。这种方法已应用于搜索Alu重复序列的互连区域以及p15A和R6K质粒的复制起点。在p15A中发现了位于RNA-1二级结构第一个茎中的类似Alu的区域以及大肠杆菌RNA聚合酶结合位点。在R6K复制起点，在串联22bp重复序列区域发现了类似Alu的重复序列。这种比较还揭示了人类Alu重复序列序列中隐藏的周期性。已经提出了一个解释所获得数据的假设。所提出的方法可作为一种揭示具有相似遗传功能的DNA序列的方法。
