早期接触白细胞介素-4通过RNA结合蛋白HuR使CD4+ T细胞中的白细胞介素-4信使核糖核酸稳定。

Early exposure to IL-4 stabilizes IL-4 mRNA in CD4+ T cells via RNA-binding protein HuR.

作者信息

Yarovinsky Timur O, Butler Noah S, Monick Martha M, Hunninghake Gary W

机构信息

Roy J. and Lucille A. Carver College of Medicine, University of Iowa and Veterans Administration Medical Center, Iowa City, IA 52242, USA.

出版信息

J Immunol. 2006 Oct 1;177(7):4426-35. doi: 10.4049/jimmunol.177.7.4426.

DOI:10.4049/jimmunol.177.7.4426

PMID:16982877

Abstract

The mechanisms regulating IL-4 mRNA stability in differentiated T cells are not known. We found that early exposure of CD4+ T cells to endogenous IL-4 increased IL-4 mRNA stability. This effect of IL-4 was mediated by the RNA-binding protein HuR. IL-4 mRNA interacted with HuR and the dominant binding site was shown within the coding region of IL-4 mRNA. Exposure of CD4+ T cells to IL-4 had no effects on HuR expression or subcellular localization, but triggered HuR binding to IL-4 mRNA. Thus, IL-4 plays a positive role in maintaining IL-4 mRNA stability in CD4+ T cells via a HuR-mediated mechanism.

摘要

调节分化T细胞中IL-4 mRNA稳定性的机制尚不清楚。我们发现，CD4+ T细胞早期暴露于内源性IL-4可增加IL-4 mRNA的稳定性。IL-4的这种作用是由RNA结合蛋白HuR介导的。IL-4 mRNA与HuR相互作用，且主要结合位点位于IL-4 mRNA的编码区内。CD4+ T细胞暴露于IL-4对HuR的表达或亚细胞定位没有影响，但会引发HuR与IL-4 mRNA的结合。因此，IL-4通过HuR介导的机制在维持CD4+ T细胞中IL-4 mRNA稳定性方面发挥积极作用。