Chieco-Bianchi L, Collavo D, Colombatti A, Biasi G
Bibl Haematol. 1975(40):613-20. doi: 10.1159/000397582.
Experiments have been performed with the aim of elucidating the nature and the extent of the in vivo interactions between murine leukemia viruses (MuLVs) and murine sarcoma virus (MSV). BALB/c and CBA mice, injected neonatally with Graffi or passage A Gross viruses (MuLV-Gi, MuLV-G), have been inoculated as young adults with murine sarcoma virus, Moloney strain (MSV-M). A higher percentage of nonregressing sarcomas appeared in these animals, sometimes accompanied simultaneously by leukemia. The immune reactivity of mice receiving MuLV-Gi at birth was found to be significantly depressed when evaluated by the hemolytic palque-forming cell (PFC) technique. However, in mice infected with MuLV-Gi and MSV-M the number of PFC ranged within the control values or slightly increased. The potentiation of MSV-M oncogenicity following infection with MuLV was studied in a more natural situation. Adult AKR mice, known to release endogenous MuLV continuously, were injected with MSV-M. The incidence of induced sarcomas was similar to that observed in control BALB/c mice inoculated with MSV-M. Moreover, tumors developed with a very long latent period. On the other hand, the great majority of tumors showed no regression and ultimately killed the host. Additional experiments, making use of immunologic manipulation of the host and Fl hybrids, suggest that the relative resistance to MSV-M oncogenesis in AKR mice is influenced by genetic and immunologic factors. MSV recovered from MSV-M-induced tumors in AKR and C58 mice was typed by highly specific mouse antisera. The results clearly showed that formation of a new MSV pseudotype occurred in vivo, the endogenous Gross virus acting as helper.
已开展实验，旨在阐明鼠白血病病毒（MuLVs）与鼠肉瘤病毒（MSV）在体内相互作用的性质和程度。新生期注射了格拉菲病毒或传代A格罗斯病毒（MuLV - Gi、MuLV - G）的BALB/c和CBA小鼠，在成年后接种了莫洛尼株鼠肉瘤病毒（MSV - M）。这些动物中出现非消退性肉瘤的比例更高，有时还会同时伴有白血病。通过溶血空斑形成细胞（PFC）技术评估时，发现出生时接受MuLV - Gi的小鼠免疫反应性显著降低。然而，感染了MuLV - Gi和MSV - M的小鼠中，PFC数量在对照值范围内或略有增加。在更自然的情况下研究了MuLV感染后MSV - M致癌性的增强情况。已知成年AKR小鼠会持续释放内源性MuLV，给它们注射了MSV - M。诱导肉瘤的发生率与接种MSV - M的对照BALB/c小鼠中观察到的相似。此外，肿瘤出现的潜伏期很长。另一方面，绝大多数肿瘤没有消退，最终导致宿主死亡。利用宿主和F1杂种的免疫操作进行的额外实验表明，AKR小鼠对MSV - M致癌作用的相对抗性受遗传和免疫因素影响。用高度特异性的小鼠抗血清对从AKR和C58小鼠的MSV - M诱导肿瘤中回收的MSV进行分型。结果清楚地表明，体内形成了一种新的MSV假型，内源性格罗斯病毒起辅助作用。
