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用于全内反射显微镜的单粒子倏逝光散射模拟

Single-particle evanescent light scattering simulations for total internal reflection microscopy.

作者信息

Helden Laurent, Eremina Elena, Riefler Norbert, Hertlein Christopher, Bechinger Clemens, Eremin Yuri, Wriedt Thomas

机构信息

Physikalisches Institut, Universität Stuttgart, Stuttgart, Germany.

出版信息

Appl Opt. 2006 Oct 1;45(28):7299-308. doi: 10.1364/ao.45.007299.

DOI:10.1364/ao.45.007299
PMID:16983418
Abstract

We simulate and measure light scattering of a micrometer-sized spherical particle suspended in solution close to a glass substrate. The model, based on the discrete sources method, is developed to describe the experimental situation of total internal reflection microscopy experiments; i.e., the particle is illuminated by an evanescent light field originating from the glass-solvent interface. In contrast to the well-established assumption of a simple exponential decay of the scattering intensity with distance, we demonstrate significant deviations for a certain range of penetration depths and polarization states of the incident light.

摘要

我们模拟并测量了悬浮在靠近玻璃基板的溶液中的微米级球形颗粒的光散射。基于离散源方法开发的模型用于描述全内反射显微镜实验的实验情况;即,颗粒由源自玻璃 - 溶剂界面的倏逝光场照明。与散射强度随距离简单指数衰减的公认假设相反,我们证明了在一定范围的穿透深度和入射光偏振态下存在显著偏差。

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