Krug A, Krynetskaya N F, Metelev V G, Cech D, Shabarova Z A
Humboldt University, Department of Chemistry, Berlin, DDR.
Biomed Biochim Acta. 1990;49(4):161-6.
Modified oligodeoxyribonucleotides containing 3'-terminal 2'-deoxy-2'-fluorouridine (UF) or 2'-fluorothymidine (TF) were successfully applied for specific RNA hydrolysis by RNase H from E. coli. The nonanucleotides d(CACCGCGCTF) and d(CACCGCGCUF) were synthesized using the phosphoramidite solid support method. The modified nucleosides were immobilized on the CPG support and provided the starting nucleoside residues. Model experiments were carried out using the 5S RNA from E. coli ribosomes and its 1-41 fragment. It was found that the use of this type of modified probes did not decrease neither the efficiency nor the specificity of the RNase H reaction.
含有3'-末端2'-脱氧-2'-氟尿苷（UF）或2'-氟胸苷（TF）的修饰寡脱氧核糖核苷酸已成功应用于大肠杆菌RNase H介导的特异性RNA水解。使用亚磷酰胺固相支持法合成了九核苷酸d（CACCGCGCTF）和d（CACCGCGCUF）。修饰核苷固定在CPG支持物上，并提供起始核苷残基。使用来自大肠杆菌核糖体的5S RNA及其1-41片段进行了模型实验。结果发现，使用这种类型的修饰探针既不降低RNase H反应的效率，也不降低其特异性。
