McMullen G L, Sattayasai N, Hibbs A R, Marzuki S
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
Biochem Int. 1990;21(2):261-9.
Antibodies to two conserved regions (residues 29-36 and 139-151) of human interferon-alpha were raised by immunizing rabbits with four short synthetic peptides coupled to carriers. The antibodies were tested for reactivity with recombinant interferon-alpha by ELISA. Despite the amino acid conservation of the two regions, there are significant variations in the reactivity of the antibodies with the IFN-alpha subtypes. The reactivity is enhanced significantly when the disulfide bonds of the interferon molecule are reduced. The results indicate that there are subtype-specific differences in the presentation of the epitopes in these conserved regions of human interferon-alpha.
通过用四种与载体偶联的短合成肽免疫兔子，制备了针对人α干扰素两个保守区域（第29 - 36位和139 - 151位氨基酸残基）的抗体。通过酶联免疫吸附测定（ELISA）检测这些抗体与重组α干扰素的反应性。尽管这两个区域的氨基酸具有保守性，但这些抗体与α干扰素各亚型的反应性存在显著差异。当干扰素分子的二硫键被还原时，反应性显著增强。结果表明，在人α干扰素这些保守区域中，表位的呈现存在亚型特异性差异。
