Maruyama K, East J L, Wagner S H, Dmochowski L
Bibl Haematol. 1975(40):85-92. doi: 10.1159/000397521.
The possible involvement of RNA tumor virus genomes in human cell transformation was investigated. Forty-nine cell cultures from neoplastic, normal, or embryo tissues were examined for transformation, following inoculation of murine leukemia virus (MuLV), feline leukemia virus (FeLV) grown in human cells, or bone marrow aspirates from leukemia patients. Five cultures exhibited transformation (1 after inoculation of MuLV grown in human cells; 4 after inoculation of human leukemic bone marrow), and 4 were established as cell lines. They were derived from giant cell tumor and fibrosarcomas. The established transformed cells formed colonies in soft agar, grew progressively in immunosuppressed mice, and carried antigens common to FeLV and MuLV. Although virus particles were not seen in these cultures, 68S RNA was detected in their media. Medium from nontransformed parent cultures also contained 68S RNA but in amounts about 15 times less than in transformed cultures. Transformed human cells passaged in mice produced both type C virus particles and 68S RNA. Antigens common to MuLV and FeLV were found in these particles. However, the results of biological and serological studies indicate their difference from conventional MuLV and FeLV. The relationship of this virus and 68S RNA found in transformed cultures remains to be determined.
对RNA肿瘤病毒基因组在人类细胞转化中可能的参与情况进行了研究。在用鼠白血病病毒（MuLV）、在人类细胞中培养的猫白血病病毒（FeLV）或白血病患者的骨髓抽吸物接种后，对来自肿瘤、正常或胚胎组织的49种细胞培养物进行了转化检测。有5种培养物表现出转化（接种在人类细胞中培养的MuLV后1种；接种人类白血病骨髓后4种），并建立了4种细胞系。它们源自巨细胞瘤和纤维肉瘤。已建立的转化细胞在软琼脂中形成集落，在免疫抑制小鼠中逐渐生长，并携带FeLV和MuLV共有的抗原。尽管在这些培养物中未观察到病毒颗粒，但在其培养基中检测到了68S RNA。未转化的亲代培养物的培养基中也含有68S RNA，但含量比转化培养物中的少约15倍。在小鼠体内传代的转化人类细胞产生了C型病毒颗粒和68S RNA。在这些颗粒中发现了MuLV和FeLV共有的抗原。然而，生物学和血清学研究结果表明它们与传统的MuLV和FeLV不同。在转化培养物中发现的这种病毒与68S RNA的关系仍有待确定。
