Dmochowski L
Recent Results Cancer Res. 1976(54):166-84. doi: 10.1007/978-3-642-80997-2_14.
Biological studies on FBJ osteosarcoma virus in tissue cultures have led to the isolation of murine sarcoma virus. Characteristic type C-MuLV particles were observed in bone tumors induced by the SD-MSV-M-virus in vitro and in vivo. The SD-MSV-M virus also induced bone tumors in rats of all strains tested, and it has a similar tumor-inducing property in hamsters. Immunoelectronmicroscopic studies showed that envelope antigens of MSV-SD virus in rat bone tumors can be distinguished from those found in hamster bone tumor cells. In tissue cultures of MSV-SD rat bone tumors, two separate cell lines have been established: one of them releases both MSV and MuLV and the other produces MuL virus only. The MuLV in this cell line acts as helper. The different interactions appear to support the concept of control mechanisms for the partial expression of genes which are responsible for neoplastic properties, virus replication, and synthesis of gs-antigens. Biochemical studies on structural rearrangement and subunit composition of RNA released from MSV-SD virus, have shown that there are two forms of the native genome RNA differing in their sedimentation coeffiiecients and in subunit composition. In human osteosarcoma tissue culture, type-C viruslike particles are found. In cocultures derived from human osteosarcoma with cells taken from the bone marrow or peripheral blood of patients with different types of leukemia, certain morphological changes are observed which resemble those induced in animal cells by RNA tumor viruses. In osteosarcomas where no cytoplasmic antigen could be proved by an immunofluorescence test, the antigen could be produced by cocultivation with antigen-positive leukemic bone marrow cells. Whole human embryo cells treated with fluid from leukemia bone marrow cultures showed the presence of the cytoplasmic antigen when tested with positive sera, but they showed no morphologic changes. In high molecular weight RNA species, sedimentation coefficients ranging from 62S to 68S are demonstrated by molecular hybridization techniques. In cross-hybridization experiments, annealing values were observed only with complementary DNA products synthesized from sarcoma viruses. Three particularly high molecular weight RNA species released from human sarcoma cell cultures showed no cross-hybridization with either the DNA product of Rauscher leukemia virus or that of Gross leukemia virus.
对FBJ骨肉瘤病毒进行的组织培养生物学研究已导致鼠肉瘤病毒的分离。在由SD-MSV-M病毒在体外和体内诱导的骨肿瘤中观察到了特征性的C型鼠白血病病毒（C-MuLV）颗粒。SD-MSV-M病毒在所有测试品系的大鼠中也诱导了骨肿瘤，并且在仓鼠中具有类似的肿瘤诱导特性。免疫电子显微镜研究表明，大鼠骨肿瘤中MSV-SD病毒的包膜抗原可与仓鼠骨肿瘤细胞中发现的包膜抗原区分开来。在MSV-SD大鼠骨肿瘤的组织培养中，已建立了两个独立的细胞系：其中一个释放MSV和MuLV，另一个仅产生MuL病毒。该细胞系中的MuLV起辅助作用。不同的相互作用似乎支持了负责肿瘤特性、病毒复制和gs抗原合成的基因部分表达的控制机制的概念。对从MSV-SD病毒释放的RNA的结构重排和亚基组成进行的生化研究表明，天然基因组RNA有两种形式，其沉降系数和亚基组成不同。在人骨肉瘤组织培养中，发现了C型病毒样颗粒。在源自人骨肉瘤与取自不同类型白血病患者骨髓或外周血的细胞的共培养物中，观察到某些形态学变化，这些变化类似于RNA肿瘤病毒在动物细胞中诱导的变化。在通过免疫荧光试验无法证明存在细胞质抗原的骨肉瘤中，通过与抗原阳性白血病骨髓细胞共培养可产生该抗原。用白血病骨髓培养物的液体处理的整个人胚细胞在用阳性血清测试时显示存在细胞质抗原，但未显示形态学变化。在高分子量RNA种类中，分子杂交技术证明沉降系数范围为62S至68S。在交叉杂交实验中，仅与从肉瘤病毒合成的互补DNA产物观察到退火值。从人肉瘤细胞培养物中释放的三种特别高分子量的RNA种类与劳舍尔白血病病毒或格罗斯白血病病毒的DNA产物均未显示交叉杂交。
