Umetskaia V N, Rozanov Iu M
Biofizika. 1990 May-Jun;35(3):399-401.
From the study of absorption and fluorescence spectra of the complex "Hoechst-33258"--DNA at different pH it is shown that AT--specific complex with DNA is formed by the neutral dye molecule, whereas the cationic state of the dye molecule forms the nonspecific complex. Possible formation of a specific complex in which the dye is bound to DNA in its major groove is discussed.
通过对“赫斯特33258”与DNA在不同pH值下的吸收光谱和荧光光谱研究表明，中性染料分子与DNA形成AT特异性复合物，而染料分子的阳离子状态形成非特异性复合物。文中还讨论了染料在DNA大沟中与DNA结合形成特异性复合物的可能性。
