Umetskaia V N, Rozanov Iu M
Biofizika. 1991 Jan-Feb;36(1):167-9.
Fluorescence spectra of the DNA-specific dye Hoehst 33258 are obtained in a number of solvents. These spectra are interpreted as superpositions of monomeric and dimeric fluorescence bands of this compound. We show that abnormal Stokes' shift in the Hoehst fluorescence occurs only for the dimer form of this dye. It is suggested that formation of the A--T-specific complex is realized by dimers of the Hoehst 33258 located in the narrow groove of DNA.
在多种溶剂中获得了DNA特异性染料Hoehst 33258的荧光光谱。这些光谱被解释为该化合物单体和二聚体荧光带的叠加。我们表明，Hoehst荧光中异常的斯托克斯位移仅发生在该染料的二聚体形式中。有人提出，A-T特异性复合物的形成是由位于DNA窄沟中的Hoehst 33258二聚体实现的。
