Su Yinghao, Shrubsole Martha J, Ness Reid M, Cai Qiuyin, Kataoka Nobuhiko, Washington Kay, Zheng Wei
Vanderbilt University Medical Center, S-1121A Medical Center North, Nashville, TN 37232-2587, USA.
Cancer Epidemiol Biomarkers Prev. 2006 Sep;15(9):1719-26. doi: 10.1158/1055-9965.EPI-05-0946.
Tissue microarray (TMA) holds promise as a high-throughput method for the analysis of biomarkers in tissue specimens. The validity and reliability of this method, however, may vary for different biomarkers in different tissue specimens.
In this study, we evaluated the validity and reliability of using TMA to assess biomarkers in colorectal adenomas.
Sixty-three consecutive patients with colorectal adenomas were recruited in this study. Two TMA blocks were constructed using four punches from each adenoma (one periphery, one deep, and two middle zones). The immunostaining of five markers (Ki-67, cyclin D1, beta-catenin, cyclooxygenase-2, and epidermal growth factor receptor) was analyzed, and the concordance between data obtained from TMAs and standard whole-tissue sections was evaluated by Spearman's correlation and kappa analysis.
Colorectal adenoma exhibited zonal, heterogeneous expression patterns for all five markers. The concordance rates for the semiquantitative evaluation of markers between data from TMAs and whole sections ranged from 87% to 93% with corresponding kappa statistics of 77% to 90%. In addition, both quantitative and semiquantitative methods were used to score TMA sections, and good correlations between these two methods were shown for all five markers with intraclass correlation coefficients ranging from 0.5 to 0.8.
Our study indicates that TMA can be used to reliably assess the expression levels of Ki-67, cyclin D1, beta-catenin, cyclooxygenase-2, and epidermal growth factor receptor in colorectal adenoma tissues.
组织微阵列（TMA）作为一种用于分析组织标本中生物标志物的高通量方法具有广阔前景。然而，该方法的有效性和可靠性可能因不同组织标本中的不同生物标志物而有所差异。
在本研究中，我们评估了使用TMA评估结直肠腺瘤中生物标志物的有效性和可靠性。
本研究招募了63例连续的结直肠腺瘤患者。使用来自每个腺瘤的四个打孔样本（一个周边区域、一个深部区域和两个中间区域）构建两个TMA块。分析了五种标志物（Ki-67、细胞周期蛋白D1、β-连环蛋白、环氧合酶-2和表皮生长因子受体）的免疫染色情况，并通过Spearman相关性分析和kappa分析评估了从TMA获得的数据与标准全组织切片数据之间的一致性。
结直肠腺瘤对所有五种标志物均表现出区域异质性表达模式。TMA数据与全组织切片数据之间标志物半定量评估的一致性率在87%至93%之间，相应的kappa统计值在77%至90%之间。此外，对TMA切片同时采用了定量和半定量方法进行评分，所有五种标志物在这两种方法之间均显示出良好的相关性，组内相关系数在0.5至0.8之间。
我们的研究表明，TMA可用于可靠评估结直肠腺瘤组织中Ki-67、细胞周期蛋白D1、β-连环蛋白、环氧合酶-2和表皮生长因子受体的表达水平。
